HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Japanese Journal of Veterinary Research >
Volume 56, Number 2 >

Expression of α-hemoglobin stabilizing protein and cellular prion protein in a subclone of murine erythroleukemia cell line MEL

Files in This Item:
56-2_p75-84.pdf318.21 kBPDFView/Open
Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.56.2.75

Title: Expression of α-hemoglobin stabilizing protein and cellular prion protein in a subclone of murine erythroleukemia cell line MEL
Authors: Otsuka, Yayoi Browse this author
Ito, Daisuke Browse this author
Katsuoka, Kayoko Browse this author
Arashiki, Nobuto Browse this author
Komatsu, Tomohiko Browse this author
Inaba, Mutsumi Browse this author →KAKEN DB
Keywords: α-hemoglobin stabilizing protein
erythroid
murine erythroleukemia
prion protein
Issue Date: Aug-2008
Publisher: The Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 56
Issue: 2
Start Page: 75
End Page: 84
Abstract: α-Hemoglobin stabilizing protein (AHSP) functions as the erythroid-specific molecular chaperon for α-globin. AHSP gene expression has been reported to be downregulated in hematopoietic tissues of animals suffering from prion diseases though the mechanism remains to be clarified. Herein, we demonstrate that MELhipod8 cells, a subclone of murine erythroleukemia (MEL) cells, have prion protein (PrPC) on the cell surface and have highly inducible expression of the AHSP and α- and β-globin genes, resembling the expression pattern of the PrP and AHSP genes in bipotential erythroid- and megakaryocyte-lineage cells followed by erythroid differentiation in normal erythropoiesis. Moreover, MELhipod8 cells exhibit greater effective erythroid differentiation with a population of hemoglobinized normoblast-like cells than that observed for the parental MEL cells. These findings suggest that MELhipod8 cells could provide a mechanism for downregulation of the AHSP gene in prion diseases.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/34675
Appears in Collections:Japanese Journal of Veterinary Research > Volume 56, Number 2

Submitter: 獣医学部図書室

Export metadata:

OAI-PMH ( junii2 , jpcoar )


 

Feedback - Hokkaido University