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Volume 56, Number 3 >

Protection by Nigella sativa against carbon tetrachloride-induced downregulation of hepatic cytochrome P450 isozymes in rats

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.56.3.119

Title: Protection by Nigella sativa against carbon tetrachloride-induced downregulation of hepatic cytochrome P450 isozymes in rats
Authors: Ibrahim, Zein S. Browse this author
Ishizuka, Mayumi Browse this author
Soliman, Mohamed Browse this author
ElBohi, Khlood Browse this author
Sobhy, Wageh Browse this author
Muzandu, Kaampwe Browse this author
Elkattawy, Azza M. Browse this author
Sakamoto, Kentaro Q. Browse this author →KAKEN DB
Fujita, Shoichi Browse this author
Keywords: carbon tetrachloride
cytochrome P450
Nigella sativa oil
Issue Date: Nov-2008
Publisher: The Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 56
Issue: 3
Start Page: 119
End Page: 128
Abstract: Nigella sativa (family Ranunculaceae) is an annual plant that has been traditionally used on the Indian subcontinent and in Middle Eastern countries. In this study, we investigated the effect of N. sativa oil on the drug-metabolizing cytochrome P450 (CYP) enzymes and whether it has a protective effect against the acute hepatotoxicity of CCl4. Intraperitoneal injection of rats with CCl4 drastically decreased CYP2E1, CYP2B, CYP3A2, CYP2C11, and CYP1A2 mRNA and protein expressions. Oral administration of 1 ml/kg N. sativa oil every day for one week prior to CCl4 injection alleviated CCl4-induced suppression of CYP2B, CYP3A2, CYP2C11, and CYP1A2. Moreover, CCl4 increased iNOS and TNFα mRNA, while N. sativa oil administration for one week prior to CCl4 injection downregulated the CCl4-induced iNOS mRNA and upregulated IL-10 mRNA. These results indicate that N. sativa oil administration has a protective effect against the CCl4-mediated suppression of hepatic CYPs and that this protective effect is partly due to the downregulation of NO production and up-regulation of the anti-inflammatory IL-10.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/35112
Appears in Collections:Japanese Journal of Veterinary Research > Volume 56, Number 3

Submitter: 獣医学部図書室

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