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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
皇居道灌濠における雌性発生ギンブナクローンの識別と分布
| Title: | 皇居道灌濠における雌性発生ギンブナクローンの識別と分布 |
| Other Titles: | Identification and distribution of gynogenetic clones in silver crucian carp Carassius langsdorfii collected from the Dokanbori moats of the Imperial Palace, Tokyo, Japan |
| Authors: | 前田, 克明1 Browse this author | | 西村, 大介2 Browse this author | | 前村, 浩隆3 Browse this author | | 森島, 輝4 Browse this author | | 張, 全啓5 Browse this author | | 海野, 徹也6 Browse this author | | 中川, 平介7 Browse this author | | 荒井, 克俊8 Browse this author →KAKEN DB |
| Authors(alt): | Maeda, Katsuaki1 | | Nishimura, Daisuke2 | | Maemura, Hirotaka3 | | Morishima, Kagayaki4 | | Zhang, Quanqi5 | | Umino, Tetsuya6 | | Nakagawa, Heisuke7 | | Arai, Katsutoshi8 |
| Keywords: | Carassius | | クローン | | 倍数体 | | 雌性発生 | | 皇居 |
| Issue Date: | 2003 |
| Publisher: | 日本水産学会 |
| Journal Title: | 日本水産学会誌 |
| Volume: | 69 |
| Issue: | 2 |
| Start Page: | 185 |
| End Page: | 191 |
| Publisher DOI: | 10.2331/suisan.69.185 |
| Abstract: | A total of 201 silver crucian carp Carassius langsdorfii were collected from the inner moats, Kami-dokanbori, Naka-dokanbori and Shimo-dokanbori of the Imperial Palace, Tokyo, Japan, in 1996, 1997 and 1998. These moats were constructed about 400 years ago and the Kami-dokanbori has been separated by a 10 m high causeway from the other moats for at least 100 years. Ploidy determination by DNA content measurements using flow cytometry revealed that all the specimens examined were categorized to 196 triploids and 5 tetraploids. All the triploids sexchecked (n=136) were female except for 6 immature individuals. All tetraploids examined (n-5) were female. Multilocus DNA fingerprinting identified 7 clonal lines (clone I-VII) based on the genetic identity between at least two samples. Individual specific DNA fingerprints were detected in eight specimens, suggesting the presence of 8 or more independent clonal lines. Among the 7 clones, clone I was the most predominant in the three Dokanbori moats, but the frequency was different among the samples examined in each year. Clone I and VI in the Imperial Palace were genetically identical to clone 8 and 6 detected in the Chugoku district, respectively. High genetic similarities between clone I triploid and four tetraploid individuals shown by DNA fingerprinting analysis suggest that these tetraploids may be produced by accidental incorporation of haploid sperm genome into gynogenetic triploid genotype. | | 1996-1998年の皇居の上,中および下道灌濠で得たギンブナ201個体は三倍体(196)と四倍体(5)であり,それらの内,性判別を行った個体はすべて雌であった。DNAフィンガープリン卜分折の結果,7クローン(I-VII)が識別され,他に8個体が個体特異的パターンを示した。各クローンの出現頻度は年と濠により異なったが,クローンIが最も優占的であった。三倍体クローンIとVIは広島県黒瀬用のクローン8と6と各々同一であった。三倍体クローンIと四倍体個体のDNAフィンガープリントは酷似し,四倍体は三倍体から生じたものと示唆された。 |
| Rights: | 本文データは学協会の許諾に基づきCiNiiから複製したものである | | © 2003 公益社団法人日本水産学会 | | © 2003 The Japanese Society of Fisheries Science |
| (Relation)isversionof: | http://ci.nii.ac.jp/naid/110003160692/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/35172 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 荒井 克俊
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