HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >

第一卵割阻止処理によるサクラマスの四倍体誘起に伴う発生異常

Files in This Item:
arai-78.pdf3.16 MBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/35174

Title: 第一卵割阻止処理によるサクラマスの四倍体誘起に伴う発生異常
Other Titles: Aberrant and arrested embryos from masu salmon eggs treated for tetraploidization by inhibition of the first cleavage
Authors: 阪尾, 寿々1 Browse this author
藤本, 貴史2 Browse this author
田中, 稔3 Browse this author
山羽, 悦郎4 Browse this author
荒井, 克俊5 Browse this author →KAKEN DB
Authors(alt): Sakao, Suzu1
Fujimoto, Takafumi2
Tanaka, Minoru3
Yamaha, Etsuro4
Arai, Katsutoshi5
Keywords: サクラマス
第一卵割阻止
四倍体
無核細胞
倍数体
モザイク
異数体
Issue Date: 2003
Publisher: 日本水産学会
Journal Title: 日本水産学会誌
Volume: 69
Issue: 5
Start Page: 738
End Page: 748
Publisher DOI: 10.2331/suisan.69.738
Abstract: Although tetraploid is important for the mass production of sterile triploids by hybridizing with diploid, its survival capacity is extremely low. In this study, we aimed to identify the cause of such a high mortality of embryos treated with hydrostatic pressure shock (700 kg/cm^2, 7 min duration, 5-7 h after fertilization at 10℃ in masu salmon. In treated eggs, cleavage rates were delayed and aberrant cell divisions were observed during the early cleavage stage. Histological observation in the blastula stage revealed frequent occurrence of aberrant blastoderms including anuclear and/or small blastomeres. Ploidy analysis of eyed and hatching embryos revealed successful production of pure tetraploid individuals. However tetraploid survivors exhibited abnormal appearances. In the groups treated at 6.5 and 7 h, no embryonic body was observed at hatching stage. Judging from the results of each treated group, the optimum timing for the inhibition of first cleavage was 6 h after fertilization, when the eggs were cytologically staged as prometaphase to metaphase of the first cleavage . Aneuploids and mosaics were also detected in treated embryos by flow cytometry. These results suggest that abnormal cleavage and blastomeres mosaicism caused by the treatment at the first cleavage give rise to high mortality.
染色体操作において第一卵割阻止処理胚の生残率は極端に低いことが知られている。本研究では四倍体誘起個体が死亡する原因を細胞学的に解明することを目的とした。通常受精後10℃の水温条件下で培養し,第一卵割を阻止する目的で受精後5時間から7時間に,700kg/cm^2,7分間の圧力処理を施した。処理胚では,初期卵割期に分裂異常,胞胚期に無核細胞,発眼期に異数体およびモザイク個体の出現が認められた。これらの異常胚出現が四倍体誘起胚の生残率を低下させる原因であると考えられた。
Rights: 本文データは学協会の許諾に基づきCiNiiから複製したものである
© 2003 公益社団法人日本水産学会
© 2003 The Japanese Society of Fisheries Science
(Relation)isversionof: http://ci.nii.ac.jp/naid/110003145584/
Type: article
URI: http://hdl.handle.net/2115/35174
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 荒井 克俊

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

 - Hokkaido University