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マツカワVerasper moseriにおける三倍体および雌性発生二倍体の誘起

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Title: マツカワVerasper moseriにおける三倍体および雌性発生二倍体の誘起
Other Titles: Induction of tripolids and gynogenetic diploids in barfin flounder Verasper moseri
Authors: 森, 立成1 Browse this author
齊藤, 節雄2 Browse this author
岸岡, 稚青3 Browse this author
荒井, 克俊4 Browse this author →KAKEN DB
Authors(alt): Mori, Tatsunari1
Saito, Setsuo2
Kishioka, Chiharu3
Arai, Katsutoshi4
Keywords: マツカワ
精子の不活化
三倍体
第二極体放出阻止
第一卵割阻止
雌性発生二倍体
低温処理
圧力処理
Issue Date: 2004
Publisher: 日本水産学会
Journal Title: 日本水産学会誌
Volume: 70
Issue: 2
Start Page: 145
End Page: 151
Publisher DOI: 10.2331/suisan.70.145
Abstract: The optimum conditions for inducing triploidy and gynogenesis by cold and pressure shock in barfin flounder Verasper moseri were examined. The sperm of barfin flounder was genetically inactivated by the dose of 10 mJ/cm^2 UV irradiation. Triploids were induced by applying cold shock treatment with -1.5℃ at 9 min after fertilization, for 90 min duration. In this condition, hatching rate relative to the control was 29.2% and triploidy rate was 90.9%. Meiotic gynogenetic diploids were obtained by fertilizing eggs with UV-irradiated sperm and subsequent cold shock (-1.5℃) at 3-9 min after fertilization for 60-90 min duration and pressure shock (650 kg/cm^2) at 7 min after fertilization for 6 min duration. Mitotic gynogenetic diploids were obtained by pressure shock (650 kg/cm^2, 6 min) and applied at 150-240 min after fertilization.
Rights: 本文データは学協会の許諾に基づきCiNiiから複製したものである
© 2004 公益社団法人日本水産学会
© 2004 The Japanese Society of Fisheries Science
(Relation)isversionof: http://ci.nii.ac.jp/naid/110003145680/
Type: article
URI: http://hdl.handle.net/2115/35175
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 荒井 克俊

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