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ゼブラフィッシュの胚盤移植による生殖系列キメラの誘導

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Title: ゼブラフィッシュの胚盤移植による生殖系列キメラの誘導
Other Titles: Germ-line chimera produced by blastoderm transplantation in zebrafish
Authors: 長井, 輝美1 Browse this author
大谷, 哲2 Browse this author
斎藤, 大樹3 Browse this author
前川, 真吾4 Browse this author
井上, 邦夫5 Browse this author
荒井, 克俊6 Browse this author →KAKEN DB
山羽, 悦郎7 Browse this author →KAKEN DB
Authors(alt): Nagai, Terumi1
Otani, Satoshi2
Saito, Taiju3
Maegawa, Shingo4
Inoue, Kunio5
Arai, Katsutoshi6
Yamaha, Etsuro7
Keywords: PGCs
始原生殖細胞
生殖系列キメラ
ゼブラフィッシュ
胚盤移植
Issue Date: 2005
Publisher: 日本水産学会
Journal Title: 日本水産学会誌
Volume: 71
Issue: 1
Start Page: 1
End Page: 9
Publisher DOI: 10.2331/suisan.71.1
Abstract: To elucidate the effects of blastoderm graft for chimeric-fish production on further development, transplantations of upper, lower or entire part of blastoderm were performed onto the animal part of host embryos during the blastula stage in zebrafish. In the case of upper, all resultant chimeric embryos developed normally, while in the lower part, many acephalic embryos appeared. When the entire blastoderm was transplanted, many resultant embryos showed normal phenotype with extra cell-mass, while a few had double axis. vas mRNA, one of the markers of PGCs, also kept the expressions in both donor and recipient blastomeres in transplantation of the entire blastoderm. Donor PGCs were frequently detected at germinal anlagen in histological sections of 6-day larvae developed from transplantations of lower and entire blastoderm, but seldom in those of the upper part. These results might suggest that graft transplantation was effective for production of germ-line chimera, but sometimes caused un-regulaory differentiation of graft cells under the community effect.
Rights: 本文データは学協会の許諾に基づきCiNiiから複製したものである
© 2005 公益社団法人日本水産学会
© 2005 The Japanese Society of Fisheries Science
Type: article
URI: http://hdl.handle.net/2115/35176
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 荒井 克俊

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