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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Genetic verification of induced gynogenesis and microsatellite–centromere mapping in the barfin flounder, Verasper moseri
| Title: | Genetic verification of induced gynogenesis and microsatellite–centromere mapping in the barfin flounder, Verasper moseri |
| Authors: | Lahrech, Zineb Browse this author | | Kishioka, Chiharu Browse this author | | Morishima, Kagayaki Browse this author | | Mori, Tatsunari Browse this author | | Saito, Setsuo Browse this author | | Arai, Katsutoshi Browse this author →KAKEN DB |
| Keywords: | Verasper moseri | | microsatellite | | gynogenesis | | genetic map | | half-tetrad analysis |
| Issue Date: | 2007 |
| Publisher: | Elsevier |
| Journal Title: | Aquaculture |
| Volume: | 272 |
| Issue: | Supplement 1 |
| Start Page: | S115 |
| End Page: | S124 |
| Publisher DOI: | 10.1016/j.aquaculture.2007.08.005 |
| Abstract: | Primer sets were newly developed for 34 polymorphic microsatellite loci in the barfin flounder Verasper moseri. Mendelian inheritance was confirmed by examining the genotypic segregation in 2 normal diploid full-sib families. All the 34 loci showed genotypic segregation according to the Mendelian manner of inheritance; in some cases, null alleles were assumed. The genotypes at 27 loci were also examined in 4 meiotic gynogenetic diploid lines produced by fertilizing eggs with UV-irradiated sperm, followed by inhibition of the second meiotic division by cold shock. The absence of paternal alleles verified the success of gynogenetic development in all 4 meiotic gynogenetic diploid lines; the proportion of heterozygous progeny of a heterozygous mother, i.e., the frequency of second division segregation (y), was used to estimate the map distance of each microsatellite locus in relation to the centromere. Marker-centromere distances were estimated to be in the range of 0 to approximately 50 centiMorgan (cM) under the assumption of complete interference. Using 8 diagnostic loci located at the telomeric region of the chromosome, complete homozygosity was confirmed in 1 mitotic gynogenetic diploid line that was produced by suppressing the first cleavage via hydrostatic pressure shock. |
| Relation: | http://www.elsevier.com/wps/find/journaldescription.cws_home/503302/description |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/35216 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 荒井 克俊
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