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Genetic verification of induced gynogenesis and microsatellite–centromere mapping in the barfin flounder, Verasper moseri

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Title: Genetic verification of induced gynogenesis and microsatellite–centromere mapping in the barfin flounder, Verasper moseri
Authors: Lahrech, Zineb Browse this author
Kishioka, Chiharu Browse this author
Morishima, Kagayaki Browse this author
Mori, Tatsunari Browse this author
Saito, Setsuo Browse this author
Arai, Katsutoshi Browse this author →KAKEN DB
Keywords: Verasper moseri
genetic map
half-tetrad analysis
Issue Date: 2007
Publisher: Elsevier
Journal Title: Aquaculture
Volume: 272
Issue: Supplement 1
Start Page: S115
End Page: S124
Publisher DOI: 10.1016/j.aquaculture.2007.08.005
Abstract: Primer sets were newly developed for 34 polymorphic microsatellite loci in the barfin flounder Verasper moseri. Mendelian inheritance was confirmed by examining the genotypic segregation in 2 normal diploid full-sib families. All the 34 loci showed genotypic segregation according to the Mendelian manner of inheritance; in some cases, null alleles were assumed. The genotypes at 27 loci were also examined in 4 meiotic gynogenetic diploid lines produced by fertilizing eggs with UV-irradiated sperm, followed by inhibition of the second meiotic division by cold shock. The absence of paternal alleles verified the success of gynogenetic development in all 4 meiotic gynogenetic diploid lines; the proportion of heterozygous progeny of a heterozygous mother, i.e., the frequency of second division segregation (y), was used to estimate the map distance of each microsatellite locus in relation to the centromere. Marker-centromere distances were estimated to be in the range of 0 to approximately 50 centiMorgan (cM) under the assumption of complete interference. Using 8 diagnostic loci located at the telomeric region of the chromosome, complete homozygosity was confirmed in 1 mitotic gynogenetic diploid line that was produced by suppressing the first cleavage via hydrostatic pressure shock.
Type: article (author version)
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 荒井 克俊

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