|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Meiotic hybridogenesis in triploid Misgurnus loach derived from a clonal lineage
| Title: | Meiotic hybridogenesis in triploid Misgurnus loach derived from a clonal lineage |
| Other Titles: | Hybridogenesis in triploid loach |
| Authors: | Morishima, Kagayaki Browse this author | | Yoshikawa, Hiroyuki Browse this author | | Arai, Katsutoshi Browse this author →KAKEN DB |
| Keywords: | Misgurnus anguillicaudatus | | asexual vertebrates | | triploid | | hybridogenesis | | clone | | microsatellite |
| Issue Date: | Jun-2008 |
| Publisher: | Nature Publishing Group |
| Journal Title: | Heredity |
| Volume: | 100 |
| Issue: | 6 |
| Start Page: | 581 |
| End Page: | 586 |
| Publisher DOI: | 10.1038/hdy.2008.17 |
| PMID: | 18382473 |
| Abstract: | Triploid loaches Misgurnus anguillicaudatus are derived from unreduced diploid gametes produced by an asexual clonal lineage that normally undergoes gynogenetic reproduction. Here, we have investigated the reproductive system of two types of triploids: the first type carried maternally inherited clonal diploid genomes and a paternally inherited haploid genome from the same population; the second type had the same clonal diploid genomes but a haploid genome from another, genetically divergent population. The germinal vesicles of oocytes from triploid females (3n=75) contained only 25 bivalents, i.e. 50 chromosomes. Flow cytometry revealed that the majority of the progeny resulting from fertilization of eggs from triploid females with normal haploid sperm were diploid. This indicates that triploid females mainly produced haploid eggs. Microsatellite analyses of the diploid progeny of triploid females showed that one allele of the clonal genotype was not transmitted to haploid eggs. Moreover, the identity of the eliminated allele differed between the two types of triploids. Our results demonstrate that there is preferential pairing of homologous chromosomes as well as the elimination of unmatched chromosomes in the course of haploid egg formation, i.e. meiotic hybridogenesis. Two distinct genomes in the clone suggest its hybrid origin. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/35324 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 荒井 克俊
|
