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Parentage assignment of stocked black sea bream Acanthopagrus schlegelii in Hiroshima Bay using microsatellite DNA markers
Title: | Parentage assignment of stocked black sea bream Acanthopagrus schlegelii in Hiroshima Bay using microsatellite DNA markers |
Authors: | Jeong, Dal-Sang Browse this author | Gonzalez, Enrique Blanco Browse this author | Morishima, Kagayaki Browse this author | Arai, Katsutoshi Browse this author →KAKEN DB | Umino, Tetsuya Browse this author |
Keywords: | Acanthopagrus schlegelii | black sea bream | genetic tagging | microsatellite DNA | parentage analysis | stock enhancement |
Issue Date: | Aug-2007 |
Publisher: | Japanese Society of Fisheries Science |
Journal Title: | Fisheries Science |
Volume: | 73 |
Issue: | 4 |
Start Page: | 823 |
End Page: | 830 |
Publisher DOI: | 10.1111/j.1444-2906.2007.01402.x |
Abstract: | The genetic contribution of 51 broodstock, comprising 29 females and 22 males, reared at Hiroshima City Marine Products Promotion Center for the production of stocked black sea bream was monitored during two consecutive years using seven microsatellite DNA loci. The high discrimination ability of these markers was reflected in the polymorphic identification content (PIC=0.831), the exclusion probability (Q∼1), and the low probability of identity index (I=3.635^−10). The total number of breeders contributing to the mating process was estimated at 32 (62.7%) in 2000 and 30 (58.8%) in 2001. On pedigree reconstruction, 69.3% of the offspring were successfully assigned to a single broodstock pair. Loss of alleles accounted for 16.9% during seed production; nevertheless, 90.9% of males and 69.0% of females participated in the mating process. Based on microsatellite genetic tagging, 58.9% of the fish sampled during the two months after release were identified as hatchery stock, presenting no significant differences from wild conspecifics in either fork length or body weight. |
Rights: | © 2007 公益社団法人日本水産学会 | © 2007 The Japanese Society of Fisheries Science |
Type: | article |
URI: | http://hdl.handle.net/2115/35581 |
Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 荒井 克俊
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