|
|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Bacterial endosymbiont of the slender pigeon louse, Columbicola columbae, allied to endosymbionts of grain weevils and tsetse flies
| Title: | Bacterial endosymbiont of the slender pigeon louse, Columbicola columbae, allied to endosymbionts of grain weevils and tsetse flies |
| Authors: | Fukatsu, Takema Browse this author | | Koga, Ryuichi Browse this author | | Smith, Wendy A. Browse this author | | Tanaka, Kohjiiro Browse this author | | Nikoh, Naruo Browse this author | | Sasaki-Fukatsu, Kayoko Browse this author | | Yoshizawa, Kazunori Browse this author →KAKEN DB | | Dale, Colin Browse this author | | Clayton, Dale H. Browse this author |
| Issue Date: | Oct-2007 |
| Publisher: | American Society for Microbiology |
| Journal Title: | Applied and Environmental Microbiology |
| Volume: | 73 |
| Issue: | 20 |
| Start Page: | 6660 |
| End Page: | 6668 |
| Publisher DOI: | 10.1128/AEM.01131-07 |
| PMID: | 17766458 |
| Abstract: | The current study focuses on a symbiotic bacterium found in the slender pigeon louse, Columbicola columbae (Insecta: Phthiraptera). Molecular phylogenetic analyses indicated that the symbiont belongs to the gamma subdivision of the class Proteobacteria and is allied to Sodalis glossinidius, the secondary symbiont of tsetse flies (Glossina spp.) and also to the primary symbiont of grain weevils (Sitophilus spp.). Relative-rate tests revealed that the symbiont of C. columbae exhibits accelerated molecular evolution in comparison with the tsetse fly symbiont and the weevil symbiont. Whole-mount in situ hybridization was used to localize the symbiont and determine infection dynamics during host development. In first- and second-instar nymphs, the symbionts were localized in the cytoplasm of oval bacteriocytes that formed small aggregates on both sides of the body cavity. In third-instar nymphs, the bacteriocytes migrated to the central body and were finally located in the anterior region of the lateral oviducts, forming conspicuous tissue formations called ovarial ampullae. In adult females, the symbionts were transmitted from the ovarial ampullae to developing oocytes in the ovarioles. In adult males, the bacteriocytes often disappeared without migration. A diagnostic PCR survey of insects collected from Japan, the United States, Australia, and Argentina detected 96.5% (109/113) infection, with a few uninfected male insects. This study provides the first microbial characterization of a bacteriocyte-associated symbiont from a chewing louse. Possible biological roles of the symbiont are discussed in relation to the host nutritional physiology associated with the feather-feeding lifestyle. |
| Rights: | Copyright © 2007 American Society for Microbiology |
| Type: | article |
| URI: | http://hdl.handle.net/2115/35630 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 吉澤 和徳
|
