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北海道大学水産科学研究彙報 = Bulletin of Fisheries Sciences, Hokkaido University >
第58巻 第3号 >

エゾバフンウニ Strongylocentrotus intermedius 消化管からタンパク質分解酵素(スブチラーゼ)の精製

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Title: エゾバフンウニ Strongylocentrotus intermedius 消化管からタンパク質分解酵素(スブチラーゼ)の精製
Other Titles: Purification of Subtilase from Short-Spined Sea Urchin Strongylocentrotus intermedius
Authors: 浦, 和寛1 Browse this author →KAKEN DB
田中, 恵梨2 Browse this author
東藤, 孝3 Browse this author →KAKEN DB
後藤, 孝弘4 Browse this author
清水, 幹博5 Browse this author →KAKEN DB
尾島, 孝男6 Browse this author →KAKEN DB
都木, 靖彰7 Browse this author →KAKEN DB
Authors(alt): Ura, Kazuhiro1
Tanaka, Eri2
Todo, Takasi3
Gotoh, Takahiro4
Shimizu, Motohiro5
Ojima, Takao6
Takagi, Yasuaki7
Keywords: Strongylocentortus intermedius
Protease
Digest organ
Issue Date: 28-Feb-2009
Publisher: 北海道大学大学院水産科学研究院
Journal Title: 北海道大学水産科学研究彙報
Journal Title(alt): Bulletin of Fisheries Sciences, Hokkaido University
Volume: 58
Issue: 3
Start Page: 21
End Page: 28
Abstract: We purified a subtilase from digestive system of short spined sea urchin Strongylocentortus intermedius by a combination of ion-exchange chromatography and gel filtration. The molecular weight of purified subtilase on SDS-PAGE under both reducing and nonreducing conditions was 35,000. Antiserum against subtilase specifically immunostained its antigen. By Western blot analysis, immunoreactive with this antibody were observed in stomach and intestine.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/36084
Appears in Collections:北海道大学水産科学研究彙報 = Bulletin of Fisheries Sciences, Hokkaido University > 第58巻 第3号

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