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Evaluation of the potency, optimal antigen level and lasting immunity of inactivated avian influenza vaccine prepared from H5N1 virus

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Title: Evaluation of the potency, optimal antigen level and lasting immunity of inactivated avian influenza vaccine prepared from H5N1 virus
Authors: Sasaki, Takashi Browse this author
Isoda, Norikazu Browse this author
Soda, Kosuke Browse this author
Sakamoto, Ryuichi Browse this author
Saijo, Kazue Browse this author
Hagiwara, Junko Browse this author
Kokumai, Norihide Browse this author
Ohgitani, Toshiaki Browse this author
Imamura, Takashi Browse this author
Sawata, Akira Browse this author
Lin, Zhifeng Browse this author
Sakoda, Yoshihiro Browse this author →KAKEN DB
Kida, Hiroshi Browse this author →KAKEN DB
Keywords: avian influenza vaccine
minimum antigen
minimum HI antibody
lasting immunity
optimal antigen level
Issue Date: Feb-2009
Publisher: The Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 56
Issue: 4
Start Page: 189
End Page: 198
Abstract: Test vaccines comprised of inactivated water-in-oil emulsions containing various antigen levels were prepared using a non-pathogenic H5N1 avian influenza (AI) virus, A/duck/Hokkaido/Vac-1/04 (H5N1). The potencies of these test vaccines were evaluated by two experiments. In the first experiment, the triangular relationship among the antigen levels of test vaccines, the hemagglutination inhibition (HI) antibody response, and the protective effect against challenge with a highly pathogenic avian influenza (HPAI) virus, A/chicken/Yamaguchi/7/04 (H5N1), was confirmed. Then lasting immunity of chickens after a single-shot vaccination was confirmed in the second experiment. As a result, complete protection after the challenge was observed in chickens immunized by test vaccines with an antigen level of 160 HA units/dose or higher. Thus, it was ascertained that the minimum antigen level in the AI vaccine was 160 HA units/dose, and the minimum HI antibody titer that could protect chickens from HPAI virus infection-related death was considered to be 1:16. Dose-dependent HI antibody responses were observed in chickens after the vaccination. Thus, 640 HA units/dose was thought to be similar to the optimal antigen level. Alternatively, the HI antibody titers of chickens, injected with the vaccine containing 640 HA units/dose, were maintained at 1:181 or higher for 100 weeks after the single-shot vaccination.
Type: bulletin (article)
Appears in Collections:Japanese Journal of Veterinary Research > Volume 56, Number 4

Submitter: 獣医学部図書室

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