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Establishment and Characterization of Seven Continuous Cell Lines from Freshwater Fish

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/38311

Title: Establishment and Characterization of Seven Continuous Cell Lines from Freshwater Fish
Authors: Fernandez, R. D. Browse this author
Yoshimizu, M. Browse this author →KAKEN DB
Kimura, T. Browse this author
Ezura, Y. Browse this author
Issue Date: Jun-1993
Publisher: American Fisheries Society
Journal Title: Journal of Aquatic Animal Health
Volume: 5
Issue: 2
Start Page: 137
End Page: 147
Publisher DOI: 10.1577/1548-8667(1993)005<0137:EACOSC>2.3.CO;2
Abstract: Seven continuous cell lines were established from salmonid and nonsalmonid fishes. Salmonid cell lines derived from rainbow trout Oncorhynchus mykiss and chum salmon O. keta were designated RTE and RTE-2 (rainbow trout embryo), RTT (rainbow trout tail), and SEH ("sake" or chum salmon embryo head). Nonsalmonid cell lines derived from pond smelt Hypomesus olidus, chevron snakehead Channa striata, and goldfish Carassius auratus were designated WF-1 ("wakasagi" fin), SHH (snakehead heart), and EPG (epithelioma papulosum of goldfish), respectively. Optimum growth for most of the cell lines was observed in Eagle's minimum essential medium buffered with sodium bicarbonate (26 mM) or a combination of sodium bicarbonate (8.9 mM) and tris (16 mM). Likewise, most of the cell lines showed optimum growth at the lowest NaCl concentration tested (0.116 M). Optimum growth temperatures ranged from 15 to 20℃ for the salmonid cell lines and from 15 to 30℃ for nonsalmonid cell lines. Except for RTT, the cell lines were heteroploid. Eleven fish viruses were used to test the susceptibility of these cell lines. Cell lines derived from salmonids developed cytopathic effects (CPE) when infected with 10 of the 11 fish viruses tested, except for RTT, which produced CPE with only 8 of the fish viruses. Six fish rhabdoviruses used in this study elicited a pronounced CPE when inoculated into nonsalmonid cell lines EPG, WF-1, and SHH. Among the new cell lines, RTE-2 showed the best potential for the isolation of fish viruses.
Type: article
URI: http://hdl.handle.net/2115/38311
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

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