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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Dental Medicine / Faculty of Dental Medicine >
Peer-reviewed Journal Articles, etc >
Bone morphogenetic protein-2 down-regulates miR-206 expression by blocking its maturation process
| Title: | Bone morphogenetic protein-2 down-regulates miR-206 expression by blocking its maturation process |
| Authors: | Sato, Mari M. Browse this author | | Nashimoto, Masayuki Browse this author | | Katagiri, Takenobu Browse this author | | Yawaka, Yasutaka Browse this author | | Tamura, Masato Browse this author →KAKEN DB |
| Keywords: | miR-206 | | miRNA processing | | bone morphogenetic protein | | Smad | | myoblasts |
| Issue Date: | 22-May-2009 |
| Publisher: | Academic Press |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 383 |
| Issue: | 1 |
| Start Page: | 125 |
| End Page: | 129 |
| Publisher DOI: | 10.1016/j.bbrc.2009.03.142 |
| PMID: | 19341710 |
| Abstract: | MicroRNAs (miRNAs) are small non-coding RNAs that are emerging as important post-transcriptional gene regulators. miR-206 is unique in that it is expressed only in skeletal muscle, including the myoblastic C2C12 cell line. In C2C12 cells, miR-206 expression was reduced dramatically after bone morphogenetic protein (BMP)-2 treatment. The down-regulation of miR-206 expression was also observed after co-transfection with constitutively-active Smad1 and Smad4, which are the intracellular signaling molecules of the BMP pathway. BMP-2 also reduced miR-206 expression in the presence of α-amanitin in a similar manner to that in the absence of α-amanitin. Moreover, the expression of pri-miR-206 was increased upon BMP-2 treatment for 6 hours compared to that in the absence of BMP-2. These results suggested that BMP-2 down-regulates miR-206 expression at the post-transcriptional level, by inhibiting the processing of pri-miR-206 into mature miR-206, and that BMP-2 could regulate miRNA biogenesis by a novel mechanism. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/38314 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 田村 正人
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