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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
蛍光標識アビジン・ビオチン抗体法のBKDキャリヤー検索への応用について : FAT間接法との比較
| Title: | 蛍光標識アビジン・ビオチン抗体法のBKDキャリヤー検索への応用について : FAT間接法との比較 |
| Other Titles: | Comparison of FITC Conjugate Avidin-Biotin Complex (ABC) Method and Indirect FAT for the Detection Rate of Renibacterium salmoninarum Antigen in Carrier Fish in BKD |
| Authors: | 吉水, 守1 Browse this author →KAKEN DB | | 紀, 栄興2 Browse this author | | 佐見, 学3 Browse this author | | 木村, 喬久4 Browse this author →KAKEN DB |
| Authors(alt): | Yoshimizu, Mamoru1 | | Ji, Rongxion2 | | Sami, Manabu3 | | Kimura, Takahisa4 |
| Issue Date: | Sep-1988 |
| Publisher: | 日本魚病学会 |
| Journal Title: | 魚病研究 |
| Journal Title(alt): | Fish pathology |
| Volume: | 23 |
| Issue: | 3 |
| Start Page: | 171 |
| End Page: | 174 |
| Abstract: | To increase the fluorescence intensity in the fluorescent antibody test (FAT), fluorescein labeled avidin-biotinylated antibody complex (ABC) was tested for the detection of Renibacterium salmoninarum antigen in the carrier fish of BKD. In comparison with indirect FAT, fluorescence intensity observed by ABC method was increased about 50% by multiscan fluorescent method using 96 well microtiter plate. The detection rate of R. salmoninarum antigens in kidney smears of masu salmon was also increased about 50% in comparison with indirect FAT. The procedure of the ABC method is as follows: A tissue smear was incubated for 30 minutes with antibody against R. salmoninarum. After several washings with phosphate buffered saline, goat anti-rabbit IgG covalently linked to biotin was added and allowed to react for 30 minutes. The preparation was washed again and a solution containing fluorescein-labeled avidin was added and allowed to react for 30 minutes. After final rinsing, the smear is mounted in glycerol buffered to pH 9.0 and observed by fluorescence microscope. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/38331 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 吉水 守
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