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Comparison of Genome Size and Synthesis of Structural Proteins of Hirame Rhabdovirus, Infectious Hematopoietic Necrosis Virus, and Viral Hemorrhagic Septicemia Virus

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Title: Comparison of Genome Size and Synthesis of Structural Proteins of Hirame Rhabdovirus, Infectious Hematopoietic Necrosis Virus, and Viral Hemorrhagic Septicemia Virus
Authors: Nishizawa, Toyohiko Browse this author →KAKEN DB
Yoshimizu, Mamoru Browse this author →KAKEN DB
Winton, James R. Browse this author
Kimura, Takahisa Browse this author →KAKEN DB
Issue Date: Jun-1991
Publisher: 日本魚病学会
Journal Title: 魚病研究
Journal Title(alt): Fish pathology
Volume: 26
Issue: 2
Start Page: 77
End Page: 81
Abstract: Genomic RNA was extracted from purified virions of hirame rhabdovirus (HRV), infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV). The full-length RNA was analyzed using formaldehyde agarose gel electrophoresis followed by ethidium bromide staining. Compared with an internal RNA size standard, all three viral genomic RNAs appeared to have identical relative mobilities and were estimated to be approximately 10.7 kilobases in length or about 3.7 megadaltons in molecular mass. Structural protein synthesis of HRV, IHNV, and VHSV was studied using cell cultures treated with actinomycin D. At 2h intervals, proteins were labeled with 35S-methionine, extracted, and analyzed by SDS-polyacrylamide gel electrophoresis and autoradiography. The five structural proteins of each of the three viruses appeared in the following order: nucleoprotein (N), matrix protein 1 (M1), matrix protein 2 (M2), glycoprotein (G), and polymerase (L) reflecting both the approximate relative abundance of each protein within infected cells and the gene order within the viral genome.
Type: article
URI: http://hdl.handle.net/2115/38333
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

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