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Detection of antibody against Aeromonas salmonicida in the serum of salmonid fish by the enzyme linked immunosorbent assay

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/38337

Title: Detection of antibody against Aeromonas salmonicida in the serum of salmonid fish by the enzyme linked immunosorbent assay
Authors: Yoshimizu, Mamoru Browse this author →KAKEN DB
Direkbusarakom, Sataporn Browse this author
Nomura, Tetsuichi Browse this author
Ezura, Yoshio Browse this author
Kimura, Takahisa Browse this author
Issue Date: Jun-1992
Publisher: 日本魚病学会
Journal Title: 魚病研究
Journal Title(alt): Fish pathology
Volume: 27
Issue: 2
Start Page: 73
End Page: 82
Abstract: Enzyme linked immunosorbent assay (ELISA) using the anti-masu Ig rabbit serum was applied to detect the antibody against Aeromonas salmonicida in the sera or salmonid fish. This ELISA method could detect the antibody in sera of masu (Oncorhynchus masou), chum (O. keta) and coho salmon (O. kisutch) immunized by A. salmonicida and could be applied to detect the antibody against A. salmonicida in sera of matured masu, pink (O. gorbuscha), chum, and kokanee salmon (O. nerka). In 1989, 89 masu and 250 chum salmon, and 59 kokanee were examined to detect antibody against A. salmonicida by ELISA and agglutination test. The number of the fish showing the positive reaction by ELISA, agglutination test and isolation of A. salmonicida were 262, 48, and 33, respectively. In 1990, 200 masu and kokanee salmon fry released from hatchery to river were tested and antibody against A. salmonicida was detected from 111 fish by ELISA, and the incidence of the fish showing the positive reaction was ranged from 4 to 90%.
Type: article
URI: http://hdl.handle.net/2115/38337
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

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