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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Purification of an Antiviral Substance Produced by Alteromonas sp. and Its Virucidal Activity against Fish Viruses
| Title: | Purification of an Antiviral Substance Produced by Alteromonas sp. and Its Virucidal Activity against Fish Viruses |
| Authors: | Myouga, Hisashi Browse this author | | Yoshimizu, Mamoru Browse this author →KAKEN DB | | Tajima, Kenichi Browse this author | | Ezura, Yoshio Browse this author |
| Keywords: | marine bacteria | | Alteromonas | | antiviral substance | | polypeptide |
| Issue Date: | 15-Mar-1995 |
| Publisher: | 日本魚病学会 |
| Journal Title: | 魚病研究 |
| Journal Title(alt): | Fish pathology |
| Volume: | 30 |
| Issue: | 1 |
| Start Page: | 15 |
| End Page: | 22 |
| Abstract: | An antiviral substance of a high molecular weight, low cytotoxity and potent virucidal activity was purified from the culture supernatant of a marine Alteromonas sp. 48HS-27. Maximum production of this antiviral substance by the strain in MCYG broth was attained by 72 h-incubation at 25℃. By the purification procedure involving ultrafiltration, precipitation with ammonium sulfate and acetone, gel filtration and native-polyacrylamide gel electrophoresis (PAGE), a polypeptide (48HS-27A) with antiviral activity was obtained at a 270-fold purification with 6.20% yield from the culture supernatant. Molecular weight of the purified 48HS-27A was estimated as approximately 52kDa by both native and sodium dodecyl sulfate (SDS) PAGE. The 50% infection inhibitory concentrations of this substance were from 0.09 to 2.51 μg/ml against one herpesvirus and five rhabdoviruses, whereas the minimal cytotoxic concentration of the substance was 144μg/ml against FHM and CHSE-214 cells. The purified 48HS-27A had proteolytic activity against casein and bovine serum albumin. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/38347 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 吉水 守
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