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Purification of an Antiviral Substance Produced by Alteromonas sp. and Its Virucidal Activity against Fish Viruses

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Title: Purification of an Antiviral Substance Produced by Alteromonas sp. and Its Virucidal Activity against Fish Viruses
Authors: Myouga, Hisashi Browse this author
Yoshimizu, Mamoru Browse this author →KAKEN DB
Tajima, Kenichi Browse this author
Ezura, Yoshio Browse this author
Keywords: marine bacteria
Alteromonas
antiviral substance
polypeptide
Issue Date: 15-Mar-1995
Publisher: 日本魚病学会
Journal Title: 魚病研究
Journal Title(alt): Fish pathology
Volume: 30
Issue: 1
Start Page: 15
End Page: 22
Abstract: An antiviral substance of a high molecular weight, low cytotoxity and potent virucidal activity was purified from the culture supernatant of a marine Alteromonas sp. 48HS-27. Maximum production of this antiviral substance by the strain in MCYG broth was attained by 72 h-incubation at 25℃. By the purification procedure involving ultrafiltration, precipitation with ammonium sulfate and acetone, gel filtration and native-polyacrylamide gel electrophoresis (PAGE), a polypeptide (48HS-27A) with antiviral activity was obtained at a 270-fold purification with 6.20% yield from the culture supernatant. Molecular weight of the purified 48HS-27A was estimated as approximately 52kDa by both native and sodium dodecyl sulfate (SDS) PAGE. The 50% infection inhibitory concentrations of this substance were from 0.09 to 2.51 μg/ml against one herpesvirus and five rhabdoviruses, whereas the minimal cytotoxic concentration of the substance was 144μg/ml against FHM and CHSE-214 cells. The purified 48HS-27A had proteolytic activity against casein and bovine serum albumin.
Type: article
URI: http://hdl.handle.net/2115/38347
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

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