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Antiviral Activity of Several Thai Traditional Herb Extracts against Fish Pathogenic Viruses

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Title: Antiviral Activity of Several Thai Traditional Herb Extracts against Fish Pathogenic Viruses
Authors: Direkbusarakom, Sataporn Browse this author
Herunsalee, Angkana Browse this author
Yoshimizu, Mamoru Browse this author →KAKEN DB
Ezura, Yoshio Browse this author
Keywords: antiviral activity
herb extract
fish virus
IHNV
IPNV
OMV
Issue Date: 15-Dec-1996
Publisher: 日本魚病学会
Journal Title: 魚病研究
Journal Title(alt): Fish pathology
Volume: 31
Issue: 4
Start Page: 209
End Page: 213
Abstract: Extracts from 18 Thai traditional herbs were prepared by boiling with ethanol under a soxhlet apparatus and virucidal activity against fish pathogenic viruses (IHNV, OMV and IPNV) was tested by plaque reduction method using CHSE-214 cells. When the viruses were exposed to herb extract at 500μg/ml before inoculation to CHSE-214 cells, all herbs showed antiviral activity against IHNV and OMV, reducing plaques by 65-100% and 20-100%, respectively. However, in the case of IPNV, no plaque reduction was observed by any herbs tested. Some kinds of the herb extracts prevented viral infection, when CHSE-214 cells were treated with 100 μg/ml of herbs for 3h before viral infection. The percent of plaque reduction above 50% was observed in 6, 8 and 6 kinds of herb extracts for IHNV, OMV and IPNV, respectively. These extracts may inhibit the viral adsorption to the cells. When 100μg/ml of the herb extracts was applied for infected cells, the percent of plaque reduction above 50% was observed in 0.8 and 5 kinds of herb extracts for IHNV, OMV and IPNV, respectively. This means that some of the herb extracts may inhibit the replication of OMV and IPNV in CHSE-214, but no herb has any effect for IHNV infection. Moreover, all of the tested herb extracts showed low toxicity to CHSE-214 cell line, the cytotoxic 50% value being 1,200-41,500μg/ml.
Type: article
URI: http://hdl.handle.net/2115/38351
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

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