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Survival of Koi Herpesvirus (KHV) in Environmental Water

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Title: Survival of Koi Herpesvirus (KHV) in Environmental Water
Other Titles: コイヘルペスウイルスKHVの環境水中における生存性
Authors: Shimizu, Tomoko1 Browse this author
Yoshida, Natsuko2 Browse this author
Kasai, Hisae3 Browse this author →KAKEN DB
Yoshimizu, Mamoru4 Browse this author →KAKEN DB
Authors(alt): 清水, 智子1
吉田, 夏子2
笠井, 久会3
吉水, 守4
Keywords: koi herpesvirus
KHV
Cyprinid herpesvirus 3
Cyprinus carpio
inactivation
Issue Date: Dec-2006
Publisher: 日本魚病学会
Journal Title: 魚病研究
Journal Title(alt): Fish Pathology
Volume: 41
Issue: 4
Start Page: 153
End Page: 157
Publisher DOI: 10.3147/jsfp.41.153
Abstract: 茨城県霞ヶ浦の湖水および底泥, 神奈川県鶴見川水系6地点および函館市内3河川5地点より水を採取し, KHVの環境中における生存性を培養細胞を用いて評価した。KHVの感染価は, 無処理の試料水中および底泥中ではウイルスを添加して3日以内に検出限界以下となったが, オートクレーブ滅菌またはろ過滅菌した試料中では, 比較的安定であった。また, 環境水中から抗KHV活性を有する細菌が分離された。これらの結果から, 宿主が存在しない環境水中でKHVは比較的速やかに不活化することが示された。
The survivability of koi herpesvirus (KHV) in environmental water and sediment was evaluated using CCB cells. Samples were collected from Ibaraki prefecture, Kanagawa prefecture and Hakodate, Hokkaido. Significant reduction in the infectious titer of KHV was observed within 3 days in intact environmental water or sediment. However, KHV infectivity remained for more than 7 days in autoclaved or filtered (0.45 mm) water. In the autoclaved water containing sediment, KHV infectivity dropped below detectable limits within 7 days after inoculation. Ten of the 147 bacterial strains from rivers in Kanagawa, and two of the 62 bacterial strains from water from, Hakodate showed anti-KHV properties. The results suggest that in the absence of hosts, KHV can be rapidly inactivated in environmental water.
Type: article
URI: http://hdl.handle.net/2115/38368
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

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