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口白症感染耐過トラフグ血清を用いた口白症関連タンパク質の検出

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Title: 口白症感染耐過トラフグ血清を用いた口白症関連タンパク質の検出
Other Titles: Detection of Kuchijirosho Associated Proteins by Western Blot with Sera of Tiger Puffer which Survived Kuchijirosho
Authors: 高見, 生雄1 Browse this author →KAKEN DB
粉川, 愉記2 Browse this author
西澤, 豊彦3 Browse this author →KAKEN DB
吉水, 守4 Browse this author →KAKEN DB
Authors(alt): Takami, Ikuo1
Kokawa, Yuki2
Nishizawa, Toyohiko3
Yoshimizu, Mamoru4
Keywords: kuchijirosho
Takifugu rubripes
tiger puffer
kuchijirosho associated protein
snout ulcer disease
Issue Date: Mar-2007
Publisher: 日本魚病学会
Journal Title: 魚病研究
Journal Title(alt): Fish Pathology
Volume: 42
Issue: 1
Start Page: 29
End Page: 34
Publisher DOI: 10.3147/jsfp.42.29
Abstract: Kuchijirosho (snout ulcer disease) is an infectious disease of tiger puffer Takifugu rubripes in Japan and Korea. Little is known about detailed characteristics on the causative agent of kuchijirosho, except it is a filterable agent. In the present study, kuchijirosho associated proteins (KAPs) with molecular weight of 100-120 kDa were detected in brain tissues of tiger puffer by western blot analysis with sera of tiger puffer which survived kuchijirosho, but not in the kidney or spleen tissues. Interestingly, KAPs existed in brain tissues of tiger puffer with no history of kuchijirosho, however, the KAPs were not recognized by those healthy fish sera. KAPs were fractionated in supernatant of brain tissue homogenate containing the kuchijirosho agent by ultra-centrifugation, while pathogenicity of the kuchijirosho agent was observed in the precipitated fraction rather than the supernatant. These results suggest that KAPs are not structural proteins of the kuchijirosho agent, and could be brain tissue proteins antigenically altered by infection of the kuchijirosho agent. It is also considered that the causative agent could be low in antigenicity to host fish because structural proteins of the causative agent was never recognized by host fish sera.
Type: article
URI: http://hdl.handle.net/2115/38373
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

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