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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
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細菌性腎臓病原因菌 Renibacterium salmoninarum の検出における PCR の限界
| Title: | 細菌性腎臓病原因菌 Renibacterium salmoninarum の検出における PCR の限界 |
| Other Titles: | Limited Availability of PCR for the Detection of Renibacterium salmoninarum, the Causative Agent of Bacterial Kidney Disease |
| Authors: | 奥田, 律子1 Browse this author | | 西澤, 豊彦2 Browse this author →KAKEN DB | | 吉水, 守3 Browse this author →KAKEN DB |
| Authors(alt): | Okuda, Ritsuko1 | | Nishizawa, Toyohiko2 | | Yoshimizu, Mamoru3 |
| Keywords: | Renibacterium salmoninarum | | bacterial kidney disease | | BKD | | PCR | | IFAT |
| Issue Date: | Mar-2008 |
| Publisher: | 日本魚病学会 |
| Journal Title: | 魚病研究 |
| Journal Title(alt): | Fish Pathology |
| Volume: | 43 |
| Issue: | 1 |
| Start Page: | 29 |
| End Page: | 33 |
| Publisher DOI: | 10.3147/jsfp.43.29 |
| Abstract: | Detection limit of PCR was investigated for Renibacterium salmoninarum, the causative agent of bacterial kidney disease, with a logarithmic growth phase. When genomic DNAs from serially diluted bacterial cells were subjected to PCR, the minimum concentration by PCR-detection with 30, 35 and 40 cycles of amplification were all 10^5 cells/mL, and the sensitivity was not improved by treatments of the bacteria with lysozyme, achromopeptidase and/or SDS. The extraction rate of genomic DNAs from the bacterial cells was approximately 3%. It was confirmed that PCR-detection limit for R. salmoninarum cells mixed with fish kidney tissues was significantly lower than that for the bacteria suspended in the medium. Moreover, there was no significant difference in the detection limit between immunofluorescence antibody technique (IFAT) and PCR. These results suggest that PCR detection should be followed by other methods such as IFAT. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/38379 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 吉水 守
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