HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >

A new rhabdovirus isolated in Japan from cultured hirame (Japanese flounder) Paralichthys olivaceus and ayu Plecoglossus altivelis

Files in This Item:
yoshimizu-37.pdf1.33 MBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/38544

Title: A new rhabdovirus isolated in Japan from cultured hirame (Japanese flounder) Paralichthys olivaceus and ayu Plecoglossus altivelis
Authors: Kimura, T. Browse this author
Yoshimizu, M. Browse this author →KAKEN DB
Gorie, S. Browse this author
Issue Date: 15-Oct-1986
Publisher: Inter-Research
Journal Title: Diseases of Aquatic Organisms
Volume: 1
Issue: 3
Start Page: 209
End Page: 217
Abstract: In March 1984, a new rhabdovirus was isolated from moribund cultured hirame (Japanese flounder) Paralichthys olivaceus and from ayu Plecoglossus altivelis fry In Hyogo Prefecture, Japan. From February through May 1985, the virus was again isolated from hirame in seawater tanks in Hyogo and Kagawa Prefecture, and in Hokkaido, Japan. At temperatures between 5 and 20℃ the virus replicated and induced cytopathic effects (CPE), which progressed to eventual cytolysis, in susceptible cell lines, including FHM, EPC, BF-2, RTG-2, STE-137, HF-1, BB, YNK, CCO, and EK-1. The CHSE-214, KO-6 and CHH-1 cell lines were refractory. Maximum virus titers of about 10^9.3 to 10^9.8 TCID50ml^[-1] were obtained in FHM or EPC cell lines, and virus replicated optimally at 15 to 20℃. Virus particles were bullet-shaped and measured 80 nm × 180 to 200 nm. The isolate was sensitive to pH 3, to diethyl ether, and to heat (50℃, 2 min). Virus did not hemagglutinate human O type erythrocytes. Viral replication was not inhibited by 10^[-4] M 5-iododeoxyuridine. Infectivity was not reduced by antisera aginst infectious hematopoietic necrosis virus (IHNV), viral hemorrhagic septicemia virus (VHSV), spring viremia of carp virus (SVCV), pike fry rhabdovirus (PFRV), eel virus-America (EVA), and eel virus-European (EVX). The viral isolate was pathogenic for rainbow trout Salmo gairdneri by injection but not for chum salmon Oncorhynchus keta, coho salmon O. kisutch, masu salmon O. masou, or ayu fry by water-borne exposure. The new virus has been named Rhabdovirus olivaceus (hirame rhabdovirus, HRV).
Rights: Copyright © 1986 Inter-Research
Relation: http://www.int-res.com/articles/dao/1/d001p209.pdf
Type: article
URI: http://hdl.handle.net/2115/38544
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

 - Hokkaido University