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Selection of brood stock candidates of barfin flounder using an ELISA system with recombinant protein of barfin flounder nervous necrosis virus

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Title: Selection of brood stock candidates of barfin flounder using an ELISA system with recombinant protein of barfin flounder nervous necrosis virus
Authors: Watanabe, Ken-ichi Browse this author
Nishizawa, Toyohiko Browse this author →KAKEN DB
Yoshimizu, Mamoru Browse this author →KAKEN DB
Keywords: Viral nervous necrosis (VNN)
Barfin flounder nervous necrosis virus (BFNNV)
Selection of brood fish
Recombinant protein
ELISA antibody titer
Issue Date: 14-Jul-2000
Publisher: Inter-Research
Journal Title: Diseases of Aquatic Organisms
Volume: 41
Issue: 3
Start Page: 219
End Page: 223
Publisher DOI: 10.3354/dao041219
Abstract: Barfin flounder nervous necrosis virus (BFNNV), the causative agent of viral nervous necrosis (VNN) of barfin flounder, is vertically transmitted from spawners to larvae. In the present study, an ELISA with a recombinant protein of BFNNV was performed for the detection of antibodies against BFNNV and applied for the selection of brood fish in order to prevent viral vertical transmissions. Brood stocks were divided into 4 groups based on ELISA antibody titers (≤10, 20, 40 and >40), and t BFNNV status of the brood stocks was determined by PCR. BFNNV was detected from the brood fish in the group with an antibody titer of >40 but not from those with titers ≤10, 20 and 40. The offspring obtained from PCR-negative brood fish pairs in ea group of ELISA antibody titers were subsequently reared for observation of VNN occurrence. VNN occurred in juveniles from 2 of 9 pairs of spawners with an antibody titer ≥40, but did not occur in spawners with an antibody titer of ≤10. Therefore, it concluded that selection of brood fish using both the PCR test and ELISA antibody titers could help prevent vertical transmission of BFNNV in larval production of barfin flounder.
Rights: © 2000 Inter-Research
Type: article
URI: http://hdl.handle.net/2115/38555
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

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