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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
病魚患部の加熱抽出液を抗原としたゲル内沈降反応によるサケ科魚類の細菌性腎臓病 (BKD) 診断について
| Title: | 病魚患部の加熱抽出液を抗原としたゲル内沈降反応によるサケ科魚類の細菌性腎臓病 (BKD) 診断について |
| Other Titles: | Serological Diagnosis of Bacterial Kidney Disease of Salmonid (BKD) : Immunodiffusion Test by Heat Stable Antigen Extracted from Infected Kidney |
| Authors: | 木村, 喬久1 Browse this author →KAKEN DB | | 絵面, 良男2 Browse this author →KAKEN DB | | 田島, 研一3 Browse this author →KAKEN DB | | 吉水, 守4 Browse this author →KAKEN DB |
| Authors(alt): | Kimura, Takahisa1 | | Ezura, Yoshio2 | | Tajima, Kenichi3 | | Yoshimizu, Mamoru4 |
| Issue Date: | Sep-1978 |
| Publisher: | 魚病研究談話会 |
| Journal Title: | 魚病研究 |
| Journal Title(alt): | Fish pathology |
| Volume: | 13 |
| Issue: | 2 |
| Start Page: | 103 |
| End Page: | 108 |
| Abstract: | An immunodiffusion test with heat stable antigen extracted from the kidney of infected fish is described as a method of diagnosis of BKD. Specific precipitin hands were formed within 24 hours when the rabbit anti-serum reacted with the extract of affected parts of the kidney containing heat stable specific BKD antigen. No precipitin bands were formed with heat extract of the kidney of healthy fish and heat extract of cells of other fish pathogenic bacteria, i.e. Aeromonas salmonicida, Vibrio anguillarum, etc. From the above findings, it was suggested that the correct diagnosis of BKD could be easily made in distant diagnostic lahoratory by the sending of heat treated field specimens. | | サケ科魚類の細菌性腎臓病（BKD）の血清学的診断法の一つとして，病魚患部の加熱抽出抗原とウサギ抗血清とのゲル内沈降反応の応用の可能性について検討して，次のような結果を得た。1．ゲル内沈降反応に関与するKD菌抗原は加熱に対して安定であり，BKDの診断に病魚患部の加熱抽出液を抗原とするゲル内沈降反応の応用が可能である。2．本方法の応用により遠隔の養魚場におけるBKDの発生についても，現場で加熱処理をしてある程度の保存性を付与した病魚患部試料の郵送などにより的確な診断が可能である。3．本方法に供する抗原液は可及的高い濃度に調製する必要があり，従って感染初期の病魚や保菌魚の検出への利用は困難と考えられる。 |
| Type: | article |
| URI: | http://hdl.handle.net/2115/38601 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 吉水 守
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