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Rapid, Simple Serological Diagnosis of Infectious Pancreatic Necrosis by Coagglutination Test Using Antibody-Sensitized Staphylococci

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Title: Rapid, Simple Serological Diagnosis of Infectious Pancreatic Necrosis by Coagglutination Test Using Antibody-Sensitized Staphylococci
Other Titles: 特異抗体感作 staphylococci を用いた coagglutination test の IPN 迅速診断への応用について
Authors: Kimura, Takahisa1 Browse this author →KAKEN DB
Yoshimizu, Mamoru2 Browse this author →KAKEN DB
Yasuda, Hiromitsu3 Browse this author
Authors(alt): 木村, 喬久1
吉水, 守2
安田, 広光3
Issue Date: Jun-1984
Publisher: 日本魚病学会
Journal Title: 魚病研究
Journal Title(alt): Fish pathology
Volume: 19
Issue: 1
Start Page: 25
End Page: 33
Publisher DOI: 10.3147/jsfp.19.25
Abstract: The specificity and rapidity of coagglutination test for the serological typing of IPNV isolated in cell cultures or for the direct detection of IPNV antigen in fish tissues were studied. Coagglutination tests were carried out with cell free virus antigens from selected viruses using Staphylococcus aureus (Cowan I) sensitized with anti-IPNV (Buhl) serum. Positive reactions occured only with cell free IPNV (Buhl) antigen, and no cross reactions were observed. The specificity of coagglutination test for rapid (less than 2 h) serological typing of IPNV was tested using the strains from North America, Japan and Europe. Staphylococci sensitized with anti-VR 299 serum gave positive reactions with the strains from North America and Japan (VR 299 type), but did not combine with the strains from Europe (Sp and Ab type). Although staphylococci sensitized with anti-Sp and Ab sera showed weak cross reactions with the strains from North America and Japan, the agglutination pattern was clearly different. The most important use of the coagglutination test for IPNV would be to detect specific IPNV antigen in the internal organs of infected fish. It was found that IPNV antigen could be detected in extracts of IPNV infected rainbow trout (Salmo gairdneri), coho salmon (Oncorhynchus kisutch) and amago salmon (Oncorhynchus rhodurus) tissues at relatively high sensitivity. The results obtained in this study using the coagglutination test for the diagnosis of IPN indicate that this rapid and simple test, which requires no special apparatus and can be performed in the field, is a valuable addition to the diagnostic methods available for detecting this disease.
特異抗体感作staphylococciを用いたcoagglutination testによる感染細胞培養液および病魚内臓抽出液中に存有するIPNウイルス抗原の検出法について,特に本法位のIPN迅速診断への応用を目的に検品を行ない次の如き結果を得た。抗IPNV(Buhl)血清感作staphylococciはIPNV(Buhl)と反応陽性を,IHNVやOMVとは陰性を示し,この反応の特異性はブロッキングテストにより確かめられた。血清タイプの異なるIPNV株間での交叉凝集試験では,抗IPNV VR299血清感作staphylococciは北米および我国分離株(VR299type)とのみ反応陽性を示し,Ab,Sp株を含むヨーロッパ株との反応は陰性であった。一方抗IPNV Ab,Sp血清感作staphylococciはVR299 type株と弱い交叉凝集反応を示したが,その凝集パターンはAb,Sp株のそれとは明らかに異なり,本法によりこれらの迅速・簡易血清型別が可能となった。ニジマス(Salmo gairdneri),ギンマス(Oncorhynchus kisutch)およびアマゴ(Oncorhynchus rhodurus) lPN病魚の内臓抽出液を抗原としたcoagglutination testでは,比較的高希釈(×2^4)試料においても90分間の反応で陽性結果を得,IPNの迅速な診断が可能となった。本法は特殊な実験器具を全く必要とせず,最終結果を得るまでにわずか2時間程を要するのみであることから,現場におけるIPNの迅速かつ簡易な診断法であると考える。
Type: article
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

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