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A Coagglutination Test with Antibody-Sensitized Staphylococci for Rapid and Simple Diagnosis of Bacterial and Viral Diseases of Fish

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Title: A Coagglutination Test with Antibody-Sensitized Staphylococci for Rapid and Simple Diagnosis of Bacterial and Viral Diseases of Fish
Authors: Yoshimizu, Mamoru Browse this author →KAKEN DB
Kimura, Takahisa Browse this author →KAKEN DB
Issue Date: Sep-1985
Publisher: 日本魚病学会
Journal Title: 魚病研究
Journal Title(alt): Fish pathology
Volume: 20
Issue: 2/3
Start Page: 243
End Page: 261
Abstract: The application of a coagglutination test for the diagnosis of diseases in fish was studied using staphylococci specifically sensitized with antibodies against the bacteria causing bacterial kidney disease (BKD), furunculosis, vibriosis and goldfish ulcer disease, and also against the virus causing infectious pancreatic necrosis (IPN). This method proved to be a simple, rapid and reliable diagnostic test suitable for use in the laboratory or field and requires no special apparatus. Procedures for this method are summarized as follows: 1. The kidney or affected tissue samples from the diseased fish are homogenized in four to nine times their volume of PBS or HANKS' BSS. If the antigen is heat stable, it is also heated in a boiling water bath for 30min. 2. The supernatant material is collected after centrifugation at 4000rpm for 20min. This may be omitted if a centrifuge is unavailable. 3. One drop of the supernatant material and one drop of antibody-sensitized staphylococci suspension are mixed on a glass slide and incubated in a wet chamber at room temperature. The slide is examined after 30, 60 and 90min. 4. If coagglutination is observed, the infected fish should be examined using another method to confirm the diagnostic results.
Type: article
URI: http://hdl.handle.net/2115/38607
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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