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Site-directed mutagenesis of possible catalytic residues of cellobiose 2-epimerase from Ruminococcus albus

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Title: Site-directed mutagenesis of possible catalytic residues of cellobiose 2-epimerase from Ruminococcus albus
Authors: Ito, Shigeaki Browse this author
Hamada, Shigeki Browse this author
Ito, Hiroyuki Browse this author
Matsui, Hirokazu Browse this author →KAKEN DB
Ozawa, Tadahiro Browse this author
Taguchi, Hidenori Browse this author
Ito, Susumu Browse this author
Keywords: Cellobiose 2-epimerase
Ruminococcus albus
Homology modeling
Site-directed mutagenesis
Epilactose
Prebiotics
Issue Date: Jul-2009
Publisher: Springer Netherlands
Journal Title: Biotechnology Letters
Volume: 31
Issue: 7
Start Page: 1065
End Page: 1071
Publisher DOI: 10.1007/s10529-009-9979-3
PMID: 19330485
Abstract: Cellobiose 2-epimerase (EC 5.1.3.11) from Ruminococcus albus (RaCE) catalyzes the reversible epimerization of cellobiose and lactose to 4-O-β-D-glucopyranosyl-D-mannose and 4-O-β-D-galactopyranosyl-D-mannose (epilactose). Based on the structure-based amino acid sequence alignment with N-acetyl-D-glucosamine 2-epimerases (EC 5.1.3.8) from porcine kidney and Anabaena sp. CH1 and on the computer-aided model building of the tertiary structure of RaCE, we performed site-directed mutagenesis of possible catalytic residues in the enzyme, and the mutants were expressed in Escherichia coli cells. It was found that R52, H243, E246, W249, M251, W304, E308, H374, and M378 were absolutely required for the activity of RaCE. F114 and W303 (and possibly R377) also contributed to catalysis. These possible catalytic protruded into or near the active-site cleft surrounded by the inner α-helices in the predicted (α/α)6 core barrel structure of RaCE.
Rights: The original publication is available at springerlink.com
Type: article (author version)
URI: http://hdl.handle.net/2115/38754
Appears in Collections:創成研究機構 (Creative Research Institution) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 伊藤 進

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