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Synergistic effect of human CycT1 and CRM1 on HIV-1 propagation in rat T cells and macrophages


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タイトル: Synergistic effect of human CycT1 and CRM1 on HIV-1 propagation in rat T cells and macrophages
著者: Okada, Hiroyuki 著作を一覧する
Zhang, Xianfeng 著作を一覧する
Ben Fofana, Ismael 著作を一覧する
Nagai, Mika 著作を一覧する
Suzuki, Hajime 著作を一覧する
Ohashi, Takashi 著作を一覧する
Shida, Hisatoshi 著作を一覧する
発行日: 2009年 5月12日
出版者: BioMed Central Ltd.
誌名: Retrovirology
巻: 6
開始ページ: 43
出版社 DOI: 10.1186/1742-4690-6-43
抄録: Background: In vivo studies of HIV-1 pathogenesis and testing of antiviral strategies have been hampered by the lack of an immunocompetent small animal model that is highly susceptible to HIV-1 infection. Although transgenic rats that express the HIV-1 receptor complex hCD4 and hCCR5 are susceptible to infection, HIV-1 replicates very poorly in these animals. To demonstrate the molecular basis for developing a better rat model for HIV-1 infection, we evaluated the effect of human CyclinT1 (hCycT1) and CRM1 (hCRM1) on Gag p24 production in rat T cells and macrophages using both established cell lines and primary cells prepared from hCycT1/hCRM1 transgenic rats. Results: Expression of hCycT1 augmented Gag production 20-50 fold in rat T cells, but had little effect in macrophages. Expression of hCRM1 enhanced Gag production 10-15 fold in macrophages, but only marginally in T cells. Expression of both factors synergistically enhanced p24 production to levels approximately 10-40% of those detected in human cells. R5 viruses produced in rat T cells and macrophages were fully infectious. Conclusion: The expression of both hCycT1 and hCRM1 appears to be fundamental to developing a rat model that supports robust propagation of HIV-1.
Rights: © 2009 Okada et al; licensee BioMed Central Ltd.
資料タイプ: article
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 志田 壽利


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