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Synergistic effect of human CycT1 and CRM1 on HIV-1 propagation in rat T cells and macrophages
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| Title: | Synergistic effect of human CycT1 and CRM1 on HIV-1 propagation in rat T cells and macrophages |
| Authors: | Okada, Hiroyuki Browse this author | | Zhang, Xianfeng Browse this author | | Ben Fofana, Ismael Browse this author | | Nagai, Mika Browse this author | | Suzuki, Hajime Browse this author | | Ohashi, Takashi Browse this author | | Shida, Hisatoshi Browse this author →KAKEN DB |
| Issue Date: | 12-May-2009 |
| Publisher: | BioMed Central Ltd. |
| Journal Title: | Retrovirology |
| Volume: | 6 |
| Start Page: | 43 |
| Publisher DOI: | 10.1186/1742-4690-6-43 |
| Abstract: | Background: In vivo studies of HIV-1 pathogenesis and testing of antiviral strategies have been hampered by the lack of an immunocompetent small animal model that is highly susceptible to HIV-1 infection. Although transgenic rats that express the HIV-1 receptor complex hCD4 and hCCR5 are susceptible to infection, HIV-1 replicates very poorly in these animals. To demonstrate the molecular basis for developing a better rat model for HIV-1 infection, we evaluated the effect of human CyclinT1 (hCycT1) and CRM1 (hCRM1) on Gag p24 production in rat T cells and macrophages using both established cell lines and primary cells prepared from hCycT1/hCRM1 transgenic rats. Results: Expression of hCycT1 augmented Gag production 20-50 fold in rat T cells, but had little effect in macrophages. Expression of hCRM1 enhanced Gag production 10-15 fold in macrophages, but only marginally in T cells. Expression of both factors synergistically enhanced p24 production to levels approximately 10-40% of those detected in human cells. R5 viruses produced in rat T cells and macrophages were fully infectious. Conclusion: The expression of both hCycT1 and hCRM1 appears to be fundamental to developing a rat model that supports robust propagation of HIV-1. |
| Rights: | © 2009 Okada et al; licensee BioMed Central Ltd. | | http://creativecommons.org/licenses/by/2.0 |
| Type: | article |
| URI: | http://hdl.handle.net/2115/38765 |
| Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 志田 壽利
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