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Enzymatic properties and the primary structure of a β-1,3-glucanase from the digestive fluid of the Pacific abalone Haliotis discus hannai

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Title: Enzymatic properties and the primary structure of a β-1,3-glucanase from the digestive fluid of the Pacific abalone Haliotis discus hannai
Authors: Kumagai, Yuya Browse this author
Ojima, Takao Browse this author →KAKEN DB
Keywords: abalone
mollusks
gastropod
β-1,3-glucanase
laminarinase
laminarin
laminarioligosaccharide
transglycosylation
Issue Date: Sep-2009
Publisher: Elsevier
Journal Title: Comparative Biochemistry and Physiology. Part B, Biochemistry and Molecular Biology
Volume: 154
Issue: 1
Start Page: 113
End Page: 120
Publisher DOI: 10.1016/j.cbpb.2009.05.005
PMID: 19446649
Abstract: A β-1,3-glucanase (EC 3.2.1.6) with a molecular mass of 33 kDa was isolated from the digestive fluid of the Pacific abalone Haliotis discus hannai by ammonium sulfate fractionation followed by conventional column chromatography. This enzyme, named HdLam33 in the present study, degraded laminarin and laminarioligosaccharides to laminaribiose and glucose with the optimal temperature and pH at 50℃ and 6.0, respectively. HdLam33 possessed transglycosylation activity, a characteristic property of glucan hydrolases that split glycoside linkage with a retaining manner. By the transglycosylation reaction of HdLam33, the laminaribiose unit in the non-reducing terminus of laminaritriose (donor substrate) was transferred to a free laminaribiose (acceptor substrate) resulting laminaritetraose and glucose. The resulted laminaritetraose was subsequently hydrolyzed by HdLam33 into two moles of glucose and one mole of laminaribiose. The primary structure of HdLam33 was analyzed by the cDNA method. The deduced amino-acid sequence of 329 residues corresponding to the catalytic domain of HdLam33 showed 56-61% amino-acid identity with those of other molluscan β-1,3-glucanases which have been identified as glycoside hydrolase family 16 enzymes.
Type: article (author version)
URI: http://hdl.handle.net/2115/38839
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 尾島 孝男

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