|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Molecular characterization and histochemical demonstration of salmon olfactory marker protein in the olfactory epithelium of lacustrine sockeye salmon (Oncorhynchus nerka)
| Title: | Molecular characterization and histochemical demonstration of salmon olfactory marker protein in the olfactory epithelium of lacustrine sockeye salmon (Oncorhynchus nerka) |
| Authors: | Kudo, H. Browse this author →KAKEN DB | | Doi, Y. Browse this author | | Ueda, H. Browse this author →KAKEN DB | | Kaeriyama, M. Browse this author →KAKEN DB |
| Keywords: | olfactory marker protein | | olfactory receptor neuron | | cloning | | in situ hybridization | | immunocytochemistry | | Pacific salmon | | Teleostei |
| Issue Date: | Sep-2009 |
| Publisher: | Elsevier |
| Journal Title: | Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology |
| Volume: | 154 |
| Issue: | 1 |
| Start Page: | 142 |
| End Page: | 150 |
| Publisher DOI: | 10.1016/j.cbpa.2009.05.123 |
| PMID: | 19501666 |
| Abstract: | Despite the importance of olfactory receptor neurons (ORNs) for homing migration, the expression of olfactory marker protein (OMP) is not well understood in ORNs of Pacific salmon (Genus Oncorhynchus). In this study, salmon OMP was characterized in the olfactory epithelia of lacustrine sockeye salmon (O. nerka) by molecular biological and histochemical techniques. Two cDNAs encoding salmon OMP were isolated and sequenced. These cDNAs both contained a coding region encoding 173 amino acid residues, and the molecular mass of the two proteins was calculated to be 19,581.17 and 19,387.11 Da, respectively. Both amino acid sequences showed marked homology (90%). The protein and nucleotide sequencing demonstrates the existence of high-level homology between salmon OMPs and those of other teleosts. By in situ hybridization using a digoxigeninlabeled salmon OMP cRNA probe, signals for salmon OMP mRNA were observed preferentially in the perinuclear regions of the ORNs. By immunohistochemistry using a specific antibody to salmon OMP, OMP-immunoreactivities were noted in the cytosol of those neurons. The present study is the first to describe cDNA cloning of OMP in salmon olfactory epithelium, and indicate that OMP is a useful molecular marker for the detection of the ORNs in Pacific salmon. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/38841 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 工藤 秀明
|
