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Development of a 3D collagen scaffold coated with multiwalled carbon nanotubes
Title: | Development of a 3D collagen scaffold coated with multiwalled carbon nanotubes |
Authors: | Hirata, Eri Browse this author | Uo, Motohiro Browse this author →KAKEN DB | Takita, Hiroko Browse this author →KAKEN DB | Akasaka, Tsukasa Browse this author | Watari, Fumio Browse this author | Yokoyama, Atsuro Browse this author |
Keywords: | carbon nanotubes | coating | collagen sponge honeycomb | 3D scaffolds | cell culture |
Issue Date: | Aug-2009 |
Publisher: | Wiley |
Journal Title: | Journal of Biomedical Materials Research. Part B, Applied Biomaterials |
Volume: | 90b |
Issue: | 2 |
Start Page: | 629 |
End Page: | 634 |
Publisher DOI: | 10.1002/jbm.b.31327 |
PMID: | 19213050 |
Abstract: | Carbon nanotubes (CNTs) have attractive biochemical properties such as strong cell adhesion and protein absorption, which are very useful for a cell cultivation scaffold. In this study, we prepared a multiwalled carbon nanotube-coated collagen sponge (MWCNT-coated sponge) to improve the surface properties of the collagen sponge, and its cell culturing properties were examined. The suface of the collagen sponge was homogeneously coated with MWCNTs by dispersion. MC3T3-E1 cells were cultured on and inside the MWCNT-coated sponge. The DNA content on the MWCNT-coated sponge after 1 week of culture was significantly higher than on an uncoated collagen sponge (p<0.05). There was no significant difference between the estimated ALP activity normalized by DNA quantity on the MWCNT-coated sponge and that on the uncoated collagen sponge which is well known as one of the best scaffolds for cell cultivation. In addition, the MWCNT-coated surface shows strong cell adhesion. Therefore, the MWCNT-coated collagen sponge is expected to be a useful 3D scaffold for cell cultivation. |
Rights: | This is the pre-peer reviewed version of the following article: Journal of Biomedical Materials Research, Part B, 90B(2), 2009, pp.629-634, which has been published in final form at http://dx.doi.org/10.1002/jbm.b.31327 |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/38894 |
Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 宇尾 基弘
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