HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
北海道大学水産科学研究彙報 = Bulletin of Fisheries Sciences, Hokkaido University >
第59巻 第2号 >

加熱損傷ウェルシュ菌の検出培地の比較と改良

Files in This Item:
59-2_001.pdf386.39 kBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/39007

Title: 加熱損傷ウェルシュ菌の検出培地の比較と改良
Other Titles: Comparison and Improvement of Detection Media for Sub-lethal Injured Clostridium perfringens
Authors: 清水, 茂雅1 Browse this author
堀口, 明日香2 Browse this author
山崎, 浩司3 Browse this author →KAKEN DB
川合, 祐史4 Browse this author →KAKEN DB
Authors(alt): Shimizu, Shigemasa1
Horiguchi, Asuka2
Yamazaki, Koji3
Kawai, Yuji4
Keywords: Clostridium perfringens
Injured cell
Recovery
Sodium pyruvate
Issue Date: 3-Aug-2009
Publisher: 北海道大学大学院水産科学研究院
Journal Title: 北海道大学水産科学研究彙報
Journal Title(alt): Bulletin of Fisheries Sciences, Hokkaido University
Volume: 59
Issue: 2
Start Page: 37
End Page: 42
Abstract: Recovery of heat-injured vegetative cells and spores of Clostridium perfringens was evaluated on selective media, CW Egg-yolk agar with kanamycin (ECW+A), Tryptose sulfite cycloserine agar(TSC+A)and modified Handford agar (mHFA). As a result of heat treatment at 54℃ for 15 min, viable counts of C. perfringens vegetative cells on a selective medium (ECW+A)was significantly less than those on non-selective media. This means C. perfringens vegetative cells should be in an injured state. Comparing of the three selective media, TSC+A was the best growth and recovery of heat-injured C. perfringens vegetative cells. Recovery on ECW+A was 1 log CFU/ml less than that on TSC+A. Supplementation of TSC+A with sodium pyruvate (0.1-0.5%) further enhanced recovery and detection of heat-injured C. perfringens vegetative cells, and its efficacy was the highest on TSC+A supplemented with 0.3% sodium pyruvate. Supplementation of TSC+A with sodium pyruvate did not affect recovery of heat-injured (95℃, 2 or 60 min) C. perfringens spores. These findings suggest that TSC+A is the most favorable medium for enumeration of C. perfringens and supplementation with sodium pyruvate improves recovery and detection of heat-injured C. perfringens.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/39007
Appears in Collections:北海道大学水産科学研究彙報 = Bulletin of Fisheries Sciences, Hokkaido University > 第59巻 第2号

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

Feedback - Hokkaido University