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Artificially induced tetraploid masu salmon have the ability to form primordial germ cells

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タイトル: Artificially induced tetraploid masu salmon have the ability to form primordial germ cells
著者: Sakao, Suzu 著作を一覧する
Fujimoto, Takafumi 著作を一覧する
Kobayashi, Terumasa 著作を一覧する
Yoshizaki, Goro 著作を一覧する
Yamaha, Etsuro 著作を一覧する
Arai, Katsutoshi 著作を一覧する
キーワード: Tetraploidy
Primordial germ cells (PGCs)
Pseudopodium
vasa
Surrogate propagation
Germ-line chimera
発行日: 2009年 7月
出版者: Springer Japan
誌名: Fisheries Science
巻: 75
号: 4
開始ページ: 993
終了ページ: 1000
出版社 DOI: 10.1007/s12562-009-0124-y
抄録: Diploid gametes generated with tetraploid animals are a stepping stone to improving techniques of chromosome manipulation. But artificially induced tetraploid individuals generally die soon after hatching. Diploid gametes could have been induced by in vivo cultures of tetraploid primordial germ cells (PGCs) through germ-line chimera. In the present study, characteristics of PGCs were studied in inviable tetraploid masu salmon, Oncorhynchus masou. Histological observation of tetraploid embryos revealed that the same or smaller numbers of PGCs were observed and they migrate into the genital ridges as did diploid PGCs during gonadogenesis. By whole mount in situ hybridization using vasa mRNA, 4-35 vasa-positive signals were detected in a pair of genital ridges of tetraploids. By cytological observation of genital ridge cell suspensions, several large round cells were observed, some of which extended pseudopodia. They also contained large nuclei and round granules in their cytoplasm, characteristics of PGCs. As the results suggest that inviable artificial tetraploids have PGCs, we expect to achieve diploid gamete production through surrogate propagation and tetraploid fish production.
Rights: The original publication is available at www.springerlink.com
©2009 公益社団法人日本水産学会 The Japanese Society of Fisheries Science
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/39100
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 荒井 克俊

 

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