|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Artificially induced tetraploid masu salmon have the ability to form primordial germ cells
| Title: | Artificially induced tetraploid masu salmon have the ability to form primordial germ cells |
| Authors: | Sakao, Suzu Browse this author | | Fujimoto, Takafumi Browse this author | | Kobayashi, Terumasa Browse this author | | Yoshizaki, Goro Browse this author | | Yamaha, Etsuro Browse this author →KAKEN DB | | Arai, Katsutoshi Browse this author →KAKEN DB |
| Keywords: | Tetraploidy | | Primordial germ cells (PGCs) | | Pseudopodium | | vasa | | Surrogate propagation | | Germ-line chimera |
| Issue Date: | Jul-2009 |
| Publisher: | Springer Japan |
| Journal Title: | Fisheries Science |
| Volume: | 75 |
| Issue: | 4 |
| Start Page: | 993 |
| End Page: | 1000 |
| Publisher DOI: | 10.1007/s12562-009-0124-y |
| Abstract: | Diploid gametes generated with tetraploid animals are a stepping stone to improving techniques of chromosome manipulation. But artificially induced tetraploid individuals generally die soon after hatching. Diploid gametes could have been induced by in vivo cultures of tetraploid primordial germ cells (PGCs) through germ-line chimera. In the present study, characteristics of PGCs were studied in inviable tetraploid masu salmon, Oncorhynchus masou. Histological observation of tetraploid embryos revealed that the same or smaller numbers of PGCs were observed and they migrate into the genital ridges as did diploid PGCs during gonadogenesis. By whole mount in situ hybridization using vasa mRNA, 4-35 vasa-positive signals were detected in a pair of genital ridges of tetraploids. By cytological observation of genital ridge cell suspensions, several large round cells were observed, some of which extended pseudopodia. They also contained large nuclei and round granules in their cytoplasm, characteristics of PGCs. As the results suggest that inviable artificial tetraploids have PGCs, we expect to achieve diploid gamete production through surrogate propagation and tetraploid fish production. |
| Rights: | © 2009 公益社団法人日本水産学会 | | © 2009 The Japanese Society of Fisheries Science | | The final publication is available at www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/39100 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 荒井 克俊
|
