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Artificially induced tetraploid masu salmon have the ability to form primordial germ cells

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Title: Artificially induced tetraploid masu salmon have the ability to form primordial germ cells
Authors: Sakao, Suzu Browse this author
Fujimoto, Takafumi Browse this author
Kobayashi, Terumasa Browse this author
Yoshizaki, Goro Browse this author
Yamaha, Etsuro Browse this author →KAKEN DB
Arai, Katsutoshi Browse this author →KAKEN DB
Keywords: Tetraploidy
Primordial germ cells (PGCs)
Surrogate propagation
Germ-line chimera
Issue Date: Jul-2009
Publisher: Springer Japan
Journal Title: Fisheries Science
Volume: 75
Issue: 4
Start Page: 993
End Page: 1000
Publisher DOI: 10.1007/s12562-009-0124-y
Abstract: Diploid gametes generated with tetraploid animals are a stepping stone to improving techniques of chromosome manipulation. But artificially induced tetraploid individuals generally die soon after hatching. Diploid gametes could have been induced by in vivo cultures of tetraploid primordial germ cells (PGCs) through germ-line chimera. In the present study, characteristics of PGCs were studied in inviable tetraploid masu salmon, Oncorhynchus masou. Histological observation of tetraploid embryos revealed that the same or smaller numbers of PGCs were observed and they migrate into the genital ridges as did diploid PGCs during gonadogenesis. By whole mount in situ hybridization using vasa mRNA, 4-35 vasa-positive signals were detected in a pair of genital ridges of tetraploids. By cytological observation of genital ridge cell suspensions, several large round cells were observed, some of which extended pseudopodia. They also contained large nuclei and round granules in their cytoplasm, characteristics of PGCs. As the results suggest that inviable artificial tetraploids have PGCs, we expect to achieve diploid gamete production through surrogate propagation and tetraploid fish production.
Rights: © 2009 公益社団法人日本水産学会
© 2009 The Japanese Society of Fisheries Science
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Type: article (author version)
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 荒井 克俊

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