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Quantitative Detection of Viable Flavobacterium psychrophilum in Chum Salmon Oncorhynchus keta by Colony Blotting and Immunostaining

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タイトル: Quantitative Detection of Viable Flavobacterium psychrophilum in Chum Salmon Oncorhynchus keta by Colony Blotting and Immunostaining
著者: Misaka, Naoyuki 著作を一覧する
Nishizawa, Toyohiko 著作を一覧する
Yoshimizu, Mamoru 著作を一覧する
キーワード: Flavobacterium psychrophilum
bacterial coldwater disease
BCWD
colony blotting
immunostaining
digital processing
発行日: 2008年 9月
出版者: 日本魚病学会 = Japanese Society of Fish Pathology
誌名: 魚病研究 = Fish Pathology
巻: 43
号: 3
開始ページ: 117
終了ページ: 123
出版社 DOI: 10.3147/jsfp.43.117
抄録: Quantitative detection of viable Flavobacterium psychrophilum, the etiological agent of bacterial coldwater disease, was evaluated by colony blotting and immunostaining. Bacterial colonies isolated from chum salmon Oncorhynchus keta ovarian fluids on a modified Cytophaga agar plate were blotted onto a nitrocellulose membrane and immunostained with antiserum against F. psychrophilum. Although the blotted colonies were strongly or weakly stained with the antiserum, blots from colonies of F. psychrophilum were distinguishable from those of other yellowish colonies by digital processing of the colony-blotted membrane photograph with an image-analyzing software. It was also confirmed that 12 strains of F. psychrophilum were all positive by the present method, while the subjected six isolates, which formed yellowish colonies but were not identified as F. psychrophilum by PCR targeting gyrB gene, and other reference six strains, F. branchiophilum, F. limicola, F. granuli, Pseudomonas flavescens, P. fluorescens, Chryseobacterium daecheongense, were all negative. From these results, the present procedure using colony blotting and immunostaining is useful for quantitative detection of viable F. psychrophilum from ovarian fluids and kidneys of chum salmon.
資料タイプ: article
URI: http://hdl.handle.net/2115/39127
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 吉水 守

 

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