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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Quantitative Detection of Viable Flavobacterium psychrophilum in Chum Salmon Oncorhynchus keta by Colony Blotting and Immunostaining
| Title: | Quantitative Detection of Viable Flavobacterium psychrophilum in Chum Salmon Oncorhynchus keta by Colony Blotting and Immunostaining |
| Authors: | Misaka, Naoyuki Browse this author | | Nishizawa, Toyohiko Browse this author →KAKEN DB | | Yoshimizu, Mamoru Browse this author →KAKEN DB |
| Keywords: | Flavobacterium psychrophilum | | bacterial coldwater disease | | BCWD | | colony blotting | | immunostaining | | digital processing |
| Issue Date: | Sep-2008 |
| Publisher: | 日本魚病学会 |
| Journal Title: | 魚病研究 |
| Journal Title(alt): | Fish Pathology |
| Volume: | 43 |
| Issue: | 3 |
| Start Page: | 117 |
| End Page: | 123 |
| Publisher DOI: | 10.3147/jsfp.43.117 |
| Abstract: | Quantitative detection of viable Flavobacterium psychrophilum, the etiological agent of bacterial coldwater disease, was evaluated by colony blotting and immunostaining. Bacterial colonies isolated from chum salmon Oncorhynchus keta ovarian fluids on a modified Cytophaga agar plate were blotted onto a nitrocellulose membrane and immunostained with antiserum against F. psychrophilum. Although the blotted colonies were strongly or weakly stained with the antiserum, blots from colonies of F. psychrophilum were distinguishable from those of other yellowish colonies by digital processing of the colony-blotted membrane photograph with an image-analyzing software. It was also confirmed that 12 strains of F. psychrophilum were all positive by the present method, while the subjected six isolates, which formed yellowish colonies but were not identified as F. psychrophilum by PCR targeting gyrB gene, and other reference six strains, F. branchiophilum, F. limicola, F. granuli, Pseudomonas flavescens, P. fluorescens, Chryseobacterium daecheongense, were all negative. From these results, the present procedure using colony blotting and immunostaining is useful for quantitative detection of viable F. psychrophilum from ovarian fluids and kidneys of chum salmon. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/39127 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 吉水 守
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