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Simple and inexpensive method for cryopreservation of fish sperm combining straw and powdered dry ice.

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/39128

Title: Simple and inexpensive method for cryopreservation of fish sperm combining straw and powdered dry ice.
Authors: Yasui, George Shigueki Browse this author
Arias-Rodriguez, Lenin Browse this author
Fujimoto, Takafumi Browse this author
Arai, Katsutoshi Browse this author →KAKEN DB
Keywords: cryopreservation
dry ice
fish
cooling rate
Misgurnus
sperm
straw
Issue Date: 1-Sep-2008
Publisher: CryoLetters
Journal Title: CryoLetters
Volume: 29
Issue: 5
Start Page: 383
End Page: 390
PMID: 18946552
Abstract: Here, we propose a simple and inexpensive method for fish sperm cryopreservation. Sperm samples of the loach Misgurnus anguillicaudatus (Teleostei: Cobitidae) were diluted 7-fold by an extender containing 63.5mM NaCl, 114mM KCl, 20mM Tris and 10% methanol. The cryogenic straws were placed in three kinds of self-made tubes which diameter was changed by commercially available materials and then immersed into powdered dry ice for 2 min and plunged into liquid nitrogen. This procedure resulted in a cooling rate at -421.4±119.84 (control), -55.8 ± 4.32 (tube 1), -40.2 ± 3.43 (tube 2) and -33.3 ± 2.09ºC/min (tube 3). In the slowest cooling rate by the tube 3, total motility (72 ± 3%), duration (146 ± 12s) and hatching rates (29 ± 04%) were higher than those by other rates. Progressive motility (83±5%) did not differ significantly from fresh samples.
Type: article
URI: http://hdl.handle.net/2115/39128
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 荒井 克俊

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