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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Simple and inexpensive method for cryopreservation of fish sperm combining straw and powdered dry ice.
| Title: | Simple and inexpensive method for cryopreservation of fish sperm combining straw and powdered dry ice. |
| Authors: | Yasui, George Shigueki Browse this author | | Arias-Rodriguez, Lenin Browse this author | | Fujimoto, Takafumi Browse this author | | Arai, Katsutoshi Browse this author →KAKEN DB |
| Keywords: | cryopreservation | | dry ice | | fish | | cooling rate | | Misgurnus | | sperm | | straw |
| Issue Date: | 1-Sep-2008 |
| Publisher: | CryoLetters |
| Journal Title: | CryoLetters |
| Volume: | 29 |
| Issue: | 5 |
| Start Page: | 383 |
| End Page: | 390 |
| PMID: | 18946552 |
| Abstract: | Here, we propose a simple and inexpensive method for fish sperm cryopreservation. Sperm samples of the loach Misgurnus anguillicaudatus (Teleostei: Cobitidae) were diluted 7-fold by an extender containing 63.5mM NaCl, 114mM KCl, 20mM Tris and 10% methanol. The cryogenic straws were placed in three kinds of self-made tubes which diameter was changed by commercially available materials and then immersed into powdered dry ice for 2 min and plunged into liquid nitrogen. This procedure resulted in a cooling rate at -421.4±119.84 (control), -55.8 ± 4.32 (tube 1), -40.2 ± 3.43 (tube 2) and -33.3 ± 2.09ºC/min (tube 3). In the slowest cooling rate by the tube 3, total motility (72 ± 3%), duration (146 ± 12s) and hatching rates (29 ± 04%) were higher than those by other rates. Progressive motility (83±5%) did not differ significantly from fresh samples. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/39128 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 荒井 克俊
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