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Ca2+ influx and phosphoinositide signalling are essential for the establishment and maintenance of cell polarity in monospores from the red alga Porphyra yezoensis

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Title: Ca2+ influx and phosphoinositide signalling are essential for the establishment and maintenance of cell polarity in monospores from the red alga Porphyra yezoensis
Authors: Li, Lin Browse this author
Saga, Naotsune Browse this author →KAKEN DB
Mikami, Koji Browse this author →KAKEN DB
Keywords: Ca2+ influx
cell polarity
cell wall
F-actin
monospore
phosphatidylinositol
phospholipase C
phospholipase D
Porphyra yezoensis
Issue Date: Aug-2009
Publisher: Oxford University Press
Journal Title: Journal of Experimental Botany
Volume: 60
Issue: 12
Start Page: 3477
End Page: 3489
Publisher DOI: 10.1093/jxb/erp183
Abstract: The asymmetrical distribution of F-actin directed by cell polarity has been observed during the migration of monospores from the red alga Porphyra yezoensis. The significance of Ca2+ influx and phosphoinositide signalling during the formation of cell polarity in migrating monospores was analysed pharmacologically. The results indicate that the inhibition of the establishment of cell polarity, as judged by the ability of F-actin to localize asymmetrically, cell wall synthesis, and development into germlings, occurred when monospores were treated with inhibitors of the Ca2+ permeable channel, phospholipase C (PLC), diacylglycerol kinase, and inositol-1,4,5-trisphosphate receptor. Moreover, it was also found that light triggered the establishment of cell polarity via photosynthetic activity but not its direction, indicating that the Ca2+ influx and PLC activation required for the establishment of cell polarity are light dependent. By contrast, inhibition of phospholipase D (PLD) prevented the migration of monospores but not the asymmetrical localization of F-actin. Taken together, these findings suggest that there is functional diversity between the PLC and PLD signalling systems in terms of the formation of cell polarity; the former being critical for the light-dependent establishment of cell polarity and the latter playing a role in the maintenance of established cell polarity.
Rights: http://creativecommons.org/licenses/bync/ 2.0/uk/
Type: article
URI: http://hdl.handle.net/2115/39136
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 三上 浩司

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