|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Phosphatidylinositol 3-kinase activity and asymmetrical accumulation of F-actin are necessary for establishment of cell polarity in the early development of monospores from the marine red alga Porphyra yezoensis
This item is licensed under:Creative Commons Attribution-NonCommercial 2.0 Generic
| Title: | Phosphatidylinositol 3-kinase activity and asymmetrical accumulation of F-actin are necessary for establishment of cell polarity in the early development of monospores from the marine red alga Porphyra yezoensis |
| Authors: | Li, Lin Browse this author | | Saga, Naotsune Browse this author | | Mikami, Koji Browse this author →KAKEN DB |
| Keywords: | Cell polarity | | cytoskeleton | | F-actin | | monospore | | phosphatidylinositol 3-kinase | | Porphyra yezoensis |
| Issue Date: | Oct-2008 |
| Publisher: | Oxford University Press |
| Journal Title: | Journal of Experimental Botany |
| Volume: | 59 |
| Issue: | 13 |
| Start Page: | 3575 |
| End Page: | 3586 |
| Publisher DOI: | 10.1093/jxb/ern207 |
| Abstract: | The polarized distribution of F-actin is important in providing the driving force for directional migration in mammalian leukocytes and Dictyostelium cells, in which compartmentation of phosphatidylinositol 3-kinase (PI3K) and phosphatidylinositol phosphatase is critical for the establishment of cell polarity. Since monospores from the red alga Porphyra yezoensis are a real example of migrating plant cells, the involvement of the cytoskeleton and PI3K was investigated during their early development. Our results indicate that the asymmetrical localization of F-actin at the leading edge is fixed by the establishment of the anterior-posterior axis in migrating monospores, which is PI3K-dependent and protein synthesis-independent. After migration, monospores adhere to the substratum and then become upright, developing into multicellular thalli via the establishment of the apical-basal axis. In this process, F-actin usually accumulates at the bottom of the basal cell and development after migration requires new protein synthesis. These findings suggest that the establishment of anterior-posterior and apical-basal axes are differentially regulated during the early development of monospores. Our results also indicate that PI3K-dependent F-actin asymmetry is evolutionally conserved in relation to the establishment of cell polarity in migrating eukaryotic cells. |
| Rights: | http://creativecommons.org/licenses/by-nc/2.0/uk/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/39139 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 三上 浩司
|
