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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
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Establishment of a neutralization test involving reporter gene-expressing virus-like particles of tick-borne encephalitis virus
| Title: | Establishment of a neutralization test involving reporter gene-expressing virus-like particles of tick-borne encephalitis virus |
| Authors: | Yoshii, Kentaro Browse this author →KAKEN DB | | Ikawa, Ayae Browse this author | | Chiba, Yumiko Browse this author | | Omori, Yuki Browse this author | | Maeda, Junko Browse this author | | Murata, Ryo Browse this author | | Kariwa, Hiroaki Browse this author →KAKEN DB | | Takashima, Ikuo Browse this author →KAKEN DB |
| Keywords: | Tick-borne encephalitis | | Virus-like particles | | Neutralization test |
| Issue Date: | Oct-2009 |
| Publisher: | Elsevier B.V. |
| Journal Title: | Journal of Virological Methods |
| Volume: | 161 |
| Issue: | 1 |
| Start Page: | 173 |
| End Page: | 176 |
| Publisher DOI: | 10.1016/j.jviromet.2009.05.016 |
| PMID: | 19481114 |
| Abstract: | Previously, a system for packaging tick-borne encephalitis virus (TBEV) subgenomic replicon RNAs into single-round infectious virus-like particles (VLPs) was developed. In the present study, VLPs were applied to measuring the levels of neutralizing antibodies against TBEV as an alternative to performing neutralization tests with live virus. As markers of VLP infection, the genes for GFP and luciferase were inserted into the TBEV replicon, which was then packaged into VLPs. The reporter genes were expressed in cells that were infected with the VLPs, and this infection was inhibited by neutralizing antibodies to TBEV. Serum samples from wild rodents were used to evaluate the neutralization test using VLPs. All the sera that were positive in the conventional neutralization test were also found to be positive in the neutralization test using VLPs, and there were highly significant correlations between the neutralization titres obtained using the native virus and those using VLPs. These results indicate that VLPs that express reporter genes represent a useful and safe alternative to conventional neutralization testing using live virus. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/39146 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 好井 健太朗
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