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Establishment of a neutralization test involving reporter gene-expressing virus-like particles of tick-borne encephalitis virus

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/39146

Title: Establishment of a neutralization test involving reporter gene-expressing virus-like particles of tick-borne encephalitis virus
Authors: Yoshii, Kentaro Browse this author →KAKEN DB
Ikawa, Ayae Browse this author
Chiba, Yumiko Browse this author
Omori, Yuki Browse this author
Maeda, Junko Browse this author
Murata, Ryo Browse this author
Kariwa, Hiroaki Browse this author →KAKEN DB
Takashima, Ikuo Browse this author →KAKEN DB
Keywords: Tick-borne encephalitis
Virus-like particles
Neutralization test
Issue Date: Oct-2009
Publisher: Elsevier B.V.
Journal Title: Journal of Virological Methods
Volume: 161
Issue: 1
Start Page: 173
End Page: 176
Publisher DOI: 10.1016/j.jviromet.2009.05.016
PMID: 19481114
Abstract: Previously, a system for packaging tick-borne encephalitis virus (TBEV) subgenomic replicon RNAs into single-round infectious virus-like particles (VLPs) was developed. In the present study, VLPs were applied to measuring the levels of neutralizing antibodies against TBEV as an alternative to performing neutralization tests with live virus. As markers of VLP infection, the genes for GFP and luciferase were inserted into the TBEV replicon, which was then packaged into VLPs. The reporter genes were expressed in cells that were infected with the VLPs, and this infection was inhibited by neutralizing antibodies to TBEV. Serum samples from wild rodents were used to evaluate the neutralization test using VLPs. All the sera that were positive in the conventional neutralization test were also found to be positive in the neutralization test using VLPs, and there were highly significant correlations between the neutralization titres obtained using the native virus and those using VLPs. These results indicate that VLPs that express reporter genes represent a useful and safe alternative to conventional neutralization testing using live virus.
Type: article (author version)
URI: http://hdl.handle.net/2115/39146
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 好井 健太朗

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