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Factors influencing efficiency of transient gene expression in the red macrophyte Porphyra yezoensis

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Title: Factors influencing efficiency of transient gene expression in the red macrophyte Porphyra yezoensis
Authors: Fukuda, Satoru Browse this author
Mikami, Koji Browse this author →KAKEN DB
Uji, Toshiki Browse this author →KAKEN DB
Park, Eun-Jeong Browse this author
Ohba, Toshiharu Browse this author
Asada, Kiyozo Browse this author
Kitade, Yukihiro Browse this author
Endo, Hirotoshi Browse this author
Kato, Ikunoshin Browse this author
Saga, Naotsune Browse this author →KAKEN DB
Keywords: Porphyra yezoensis
Codon usage
β-Glucuronidase (EC 3.2.1.21)
Particle bombardment
Transient gene expression
Issue Date: Mar-2008
Publisher: Elsevier
Journal Title: Plant Science : an international journal of experimental plant biology
Volume: 174
Issue: 3
Start Page: 329
End Page: 339
Publisher DOI: 10.1016/j.plantsci.2007.12.006
Abstract: The marine red alga Porphyra yezoensis has been proposed as a model plant for physiological and genetic studies in seaweeds because of its biological and economical importance. However, the progress of molecular biological studies using gene transfection and genetic transformation systems has been hindered by difficulties in the expression of foreign genes in P. yezoensis cells. To overcome this situation, we developed a transient gene expression system to monitor gene expression in P. yezoensis cells. An artificial υ-glucuronidase (GUS) coding region was synthesized to adapt it to the codon usage of P. yezoensis (PyGUS) and then evaluated for efficiency as a reporter of transient gene expression by particle bombardment. We also demonstrated the importance of using the promoter of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from P. yezoensis for efficient expression of PyGUS, because the cauliflower mosaic virus (CaMV) 35S promoter, which has been successfully used for monitoring gene expression in nuclei and chloroplasts of higher plants, was less active in P. yezoensis cells. Therefore, the lack of knowledge about differences in the regulatory machinery of gene expression between P. yezoensis and terrestrial plants seems to be why experimental systems for monitoring gene expression were previously not developed in P. yezoensis. Establishment of the transient gene expression system in P. yezoensis could facilitate biotechnological developments in this organism.
Type: article (author version)
URI: http://hdl.handle.net/2115/39214
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 三上 浩司

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