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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Factors influencing efficiency of transient gene expression in the red macrophyte Porphyra yezoensis
| Title: | Factors influencing efficiency of transient gene expression in the red macrophyte Porphyra yezoensis |
| Authors: | Fukuda, Satoru Browse this author | | Mikami, Koji Browse this author →KAKEN DB | | Uji, Toshiki Browse this author →KAKEN DB | | Park, Eun-Jeong Browse this author | | Ohba, Toshiharu Browse this author | | Asada, Kiyozo Browse this author | | Kitade, Yukihiro Browse this author | | Endo, Hirotoshi Browse this author | | Kato, Ikunoshin Browse this author | | Saga, Naotsune Browse this author →KAKEN DB |
| Keywords: | Porphyra yezoensis | | Codon usage | | β-Glucuronidase (EC 3.2.1.21) | | Particle bombardment | | Transient gene expression |
| Issue Date: | Mar-2008 |
| Publisher: | Elsevier |
| Journal Title: | Plant Science : an international journal of experimental plant biology |
| Volume: | 174 |
| Issue: | 3 |
| Start Page: | 329 |
| End Page: | 339 |
| Publisher DOI: | 10.1016/j.plantsci.2007.12.006 |
| Abstract: | The marine red alga Porphyra yezoensis has been proposed as a model plant for physiological and genetic studies in seaweeds because of its biological and economical importance. However, the progress of molecular biological studies using gene transfection and genetic transformation systems has been hindered by difficulties in the expression of foreign genes in P. yezoensis cells. To overcome this situation, we developed a transient gene expression system to monitor gene expression in P. yezoensis cells. An artificial υ-glucuronidase (GUS) coding region was synthesized to adapt it to the codon usage of P. yezoensis (PyGUS) and then evaluated for efficiency as a reporter of transient gene expression by particle bombardment. We also demonstrated the importance of using the promoter of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from P. yezoensis for efficient expression of PyGUS, because the cauliflower mosaic virus (CaMV) 35S promoter, which has been successfully used for monitoring gene expression in nuclei and chloroplasts of higher plants, was less active in P. yezoensis cells. Therefore, the lack of knowledge about differences in the regulatory machinery of gene expression between P. yezoensis and terrestrial plants seems to be why experimental systems for monitoring gene expression were previously not developed in P. yezoensis. Establishment of the transient gene expression system in P. yezoensis could facilitate biotechnological developments in this organism. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/39214 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 三上 浩司
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