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Growth promotion and cell binding ability of bovine lactoferrin to Bifidobacterium longum

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Title: Growth promotion and cell binding ability of bovine lactoferrin to Bifidobacterium longum
Authors: Rahman, Md. Morshedur Browse this author
Kim, Woan-Sub Browse this author
Ito, Toshiaki Browse this author
Kumura, Haruto Browse this author →KAKEN DB
Shimazaki, Kei-ichi Browse this author
Keywords: Bifidobacterium longum
Bovine lactoferrin
Lactoferrin-binding protein
Issue Date: Aug-2009
Publisher: Elsevier Ltd.
Journal Title: Anaerobe
Volume: 15
Issue: 4
Start Page: 133
End Page: 137
Publisher DOI: 10.1016/j.anaerobe.2009.01.003
PMID: 19655425
Abstract: Lactoferrin, a major whey protein of human milk, is considered as growth promoter for bifidobacteria, the predominant microorganisms of human intestine. In the present study, in vitro growth promotion and cell binding ability of bovine lactoferrin to several strains of Bifidobacterium longum have been demonstrated. A dose-dependent as well as strain-dependent growth promotion effect by lactoferrin was observed. Cell binding ability of lactoferrin was inspected under an inverted confocal laser scanning microscope by incubation bacterial cells with biotinylated bovine lactoferrin and FITC-conjugated avidin. Fluorescence staining showed bovine lactoferrin binding to ail tested strains. A lactoferrin-binding protein with a molecular weight of approximately 67 kDa was also detected in the extracted membrane and cytosolic fraction of each B. longum strain by far-Western blot technique using biotinylated lactoferrin and horseradish peroxidase-conjugated streptavidin. Based on these results, we suggest that existence of lactoferrin-binding protein could be a common characteristic in bifidobacteria. It can also be hypothesized that lactoferrin-binding protein in bifidobacteria is not only involved in growth stimulation mechanism but also could play different roles.
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 島崎 敬一

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