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Main microorganisms involved in the fermentation of Ugandan ghee

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Title: Main microorganisms involved in the fermentation of Ugandan ghee
Authors: Ongol, Martin Patrick Browse this author
Asano, Kozo Browse this author →KAKEN DB
Keywords: Acetic acid bacteria
Lactic acid bacteria
Yeasts
Ghee
PCR-DGGE
Traditional milk fermentation
Issue Date: 15-Aug-2009
Publisher: Elsevier B.V.
Journal Title: International Journal of Food Microbiology
Volume: 133
Issue: 3
Start Page: 286
End Page: 291
Publisher DOI: 10.1016/j.ijfoodmicro.2009.06.003
PMID: 19577815
Abstract: Mashita, a traditional fermented butter-like product is widely produced in western Uganda. However, no detailed studies have been done to identify the microorganisms involved in mashita fermentation. Therefore, the aim of this study was to identify the microorganisms involved in mashita fermentation using culture-dependent and culture-independent techniques. The most commonly identified strains of lactic acid bacteria (LAB) in mashita using culture-dependent techniques were Lactobacillus paracasei, Lactobacillus helveticus, Lactobacillus plantarum and Lactobacillus perolens constituting 37.3%, 10.1%, 8.1% and 7.7% of total bacterial colonies isolated respectively. L. paracasei was the only bacterial species identified in all mashita samples. PCR-DGGE results indicated that acetic acid bacteria (AAB) and LAB were the dominant bacterial groups in mashita. Acetobacter aceti, Acetobacter lovaniensis, Acetobacter orientalis and Acetobacter pasteurianus were the main species of AAB identified in the mashita whereas Bifidobacterium sp., Enterococcus faecium, Lactobacillus brevis, Lactobacillus helveticus, Lactobacillus acetotolerans, Lactobacillus sp., Lactococcus raffinolactis, Lactococcus sp. and Streptococcus salivarius as the main strains of LAB in mashita. PCR-DGGE of the D1 region of yeasts showed that Brettanomyces custersianus, Candida silvae, Geotrichum candidum, Issatchenkia occidentalis, Issatchenkia orientalis, Kluyveromyces marxianus, Saccharomyces cerevisiae, and Trichosporon asahii were part of the mashita microbial community. These results indicate that AAB, LAB and yeasts are involved in mashita fermentation.
Type: article (author version)
URI: http://hdl.handle.net/2115/39221
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 浅野 行蔵

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