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Isolation and characterization of a novel chondroitin sulfate from squid liver integument rich in N-acetylgalactosamine(4,6-disulfate) and glucuronate(3-sulfate) residues

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タイトル: Isolation and characterization of a novel chondroitin sulfate from squid liver integument rich in N-acetylgalactosamine(4,6-disulfate) and glucuronate(3-sulfate) residues
著者: Shetty, Ajaya Kumar 著作を一覧する
Kobayashi, Takanari 著作を一覧する
Mizumoto, Shuji 著作を一覧する
Narumi, Masaki 著作を一覧する
Kudo, Yoshiaki 著作を一覧する
Yamada, Shuhei 著作を一覧する
Sugahara, Kazuyuki 著作を一覧する
キーワード: Chondroitin sulfate
Glycosaminoglycan
Growth factor
Neurite outgrowth
Squid liver
Surface plasmon resonance
発行日: 2009年 8月17日
出版者: Elsevier Ltd.
誌名: Carbohydrate Research
巻: 344
号: 12
開始ページ: 1526
終了ページ: 1532
出版社 DOI: 10.1016/j.carres.2009.02.029
抄録: Novel chondroitin sulfate (CS) chains with an average molecular mass of 79.6 kDa were purified from squid liver integument. A compositional analysis of the CS chains using chondroitinases (CSases) ABC and AC-I revealed a range of variably sulfated disaccharides with GlcAβ1-3GalNAc(6-O-sulfate), GlcAβ1-3GalNAc(4-O-sulfate), and GlcAβ1-3GalNAc(4,6-O-disulfate) as the major ones, significant amounts of rare 3-O-sulfated GlcA-containing disaccharides, and a small amount of nonsulfated GlcAβ1-3GalNAc. The CS chains exhibited neurite outgrowth-promoting activity toward embryonic mouse hippocampal neurons, which was abolished completely by digestion with CSase ABC or AC-I. Consequently, whether these CS chains interact with heparin-binding growth factors was tested in a BIAcore system. All of the growth factors exhibited concentration-dependent and specific binding. CS chains from squid liver integument, with their unique composition and strong biological activities, may be a good candidate for therapeutic application.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/39341
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 山田 修平

 

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