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Isolation and characterization of a novel chondroitin sulfate from squid liver integument rich in N-acetylgalactosamine(4,6-disulfate) and glucuronate(3-sulfate) residues
Title: | Isolation and characterization of a novel chondroitin sulfate from squid liver integument rich in N-acetylgalactosamine(4,6-disulfate) and glucuronate(3-sulfate) residues |
Authors: | Shetty, Ajaya Kumar Browse this author | Kobayashi, Takanari Browse this author | Mizumoto, Shuji Browse this author | Narumi, Masaki Browse this author | Kudo, Yoshiaki Browse this author | Yamada, Shuhei Browse this author →KAKEN DB | Sugahara, Kazuyuki Browse this author →KAKEN DB |
Keywords: | Chondroitin sulfate | Glycosaminoglycan | Growth factor | Neurite outgrowth | Squid liver | Surface plasmon resonance |
Issue Date: | 17-Aug-2009 |
Publisher: | Elsevier Ltd. |
Journal Title: | Carbohydrate Research |
Volume: | 344 |
Issue: | 12 |
Start Page: | 1526 |
End Page: | 1532 |
Publisher DOI: | 10.1016/j.carres.2009.02.029 |
PMID: | 19344892 |
Abstract: | Novel chondroitin sulfate (CS) chains with an average molecular mass of 79.6 kDa were purified from squid liver integument. A compositional analysis of the CS chains using chondroitinases (CSases) ABC and AC-I revealed a range of variably sulfated disaccharides with GlcAβ1-3GalNAc(6-O-sulfate), GlcAβ1-3GalNAc(4-O-sulfate), and GlcAβ1-3GalNAc(4,6-O-disulfate) as the major ones, significant amounts of rare 3-O-sulfated GlcA-containing disaccharides, and a small amount of nonsulfated GlcAβ1-3GalNAc. The CS chains exhibited neurite outgrowth-promoting activity toward embryonic mouse hippocampal neurons, which was abolished completely by digestion with CSase ABC or AC-I. Consequently, whether these CS chains interact with heparin-binding growth factors was tested in a BIAcore system. All of the growth factors exhibited concentration-dependent and specific binding. CS chains from squid liver integument, with their unique composition and strong biological activities, may be a good candidate for therapeutic application. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/39341 |
Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 山田 修平
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