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Isolation and characterization of a novel chondroitin sulfate from squid liver integument rich in N-acetylgalactosamine(4,6-disulfate) and glucuronate(3-sulfate) residues

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Title: Isolation and characterization of a novel chondroitin sulfate from squid liver integument rich in N-acetylgalactosamine(4,6-disulfate) and glucuronate(3-sulfate) residues
Authors: Shetty, Ajaya Kumar Browse this author
Kobayashi, Takanari Browse this author
Mizumoto, Shuji Browse this author
Narumi, Masaki Browse this author
Kudo, Yoshiaki Browse this author
Yamada, Shuhei Browse this author →KAKEN DB
Sugahara, Kazuyuki Browse this author →KAKEN DB
Keywords: Chondroitin sulfate
Glycosaminoglycan
Growth factor
Neurite outgrowth
Squid liver
Surface plasmon resonance
Issue Date: 17-Aug-2009
Publisher: Elsevier Ltd.
Journal Title: Carbohydrate Research
Volume: 344
Issue: 12
Start Page: 1526
End Page: 1532
Publisher DOI: 10.1016/j.carres.2009.02.029
PMID: 19344892
Abstract: Novel chondroitin sulfate (CS) chains with an average molecular mass of 79.6 kDa were purified from squid liver integument. A compositional analysis of the CS chains using chondroitinases (CSases) ABC and AC-I revealed a range of variably sulfated disaccharides with GlcAβ1-3GalNAc(6-O-sulfate), GlcAβ1-3GalNAc(4-O-sulfate), and GlcAβ1-3GalNAc(4,6-O-disulfate) as the major ones, significant amounts of rare 3-O-sulfated GlcA-containing disaccharides, and a small amount of nonsulfated GlcAβ1-3GalNAc. The CS chains exhibited neurite outgrowth-promoting activity toward embryonic mouse hippocampal neurons, which was abolished completely by digestion with CSase ABC or AC-I. Consequently, whether these CS chains interact with heparin-binding growth factors was tested in a BIAcore system. All of the growth factors exhibited concentration-dependent and specific binding. CS chains from squid liver integument, with their unique composition and strong biological activities, may be a good candidate for therapeutic application.
Type: article (author version)
URI: http://hdl.handle.net/2115/39341
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山田 修平

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