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Epstein-Barr virus (EBV)-encoded small RNA is released from EBV-infected cells and activates signaling from toll-like receptor 3

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タイトル: Epstein-Barr virus (EBV)-encoded small RNA is released from EBV-infected cells and activates signaling from toll-like receptor 3
著者: Iwakiri, Dai 著作を一覧する
Zhou, Li 著作を一覧する
Samanta, Mrinal 著作を一覧する
Matsumoto, Misako 著作を一覧する
Ebihara, Takashi 著作を一覧する
Seya, Tsukasa 著作を一覧する
Imai, Shosuke 著作を一覧する
Fujieda, Mikiya 著作を一覧する
Kawa, Keisei 著作を一覧する
Takada, Kenzo 著作を一覧する
発行日: 2009年 9月28日
出版者: Rockefeller University Press
誌名: Journal of Experimental Medicine
巻: 206
号: 10
開始ページ: 2091
終了ページ: 2099
出版社 DOI: 10.1084/jem.20081761
抄録: Epstein-Barr virus-encoded small RNA (EBER) is nonpolyadenylated, noncoding RNA that forms stem-loop structure by intermolecular base-pairing, giving rise to double-stranded RNA (dsRNA)-like molecules, and exists abundantly in EBV-infected cells. Here, we report that EBER induces signaling from the Toll-like receptor 3 (TLR3), which is a sensor of viral double-stranded RNA (dsRNA) and induces type I IFN and proinflammatory cytokines. A substantial amount of EBER, which was sufficient to induce signaling from TLR3, was released from EBV-infected cells, and the majority of the released EBER existed as a complex with a cellular EBER-binding protein La, suggesting that EBER was released from the cells by active secretion of La. Sera from patients with infectious mononucleosis (IM), chronic active EBV infection (CAEBV), and EBV-associated hemophagocytic lymphohistiocytosis (EBV-HLH), whose general symptoms are caused by proinflammatory cytokines contained EBER, and addition of RNA purified from the sera into culture medium induced signaling from TLR3 in EBV-transformed lymphocytes and peripheral mononuclear cells. Furthermore, DCs treated with EBER showed mature phenotype and antigen presentation capacity. These findings suggest that EBER, which is released from EBV-infected cells, is responsible for immune activation by EBV, inducing type I IFN and proinflammatory cytokines. EBER-induced activation of innate immunity would account for immunopathologic diseases caused by active EBV infection.
Rights: © 2009 Iwakiri et al.
資料タイプ: article
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 高田 賢藏


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