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An ultramarine fluorescent protein with increased photostability and pH insensitivity
| Title: | An ultramarine fluorescent protein with increased photostability and pH insensitivity |
| Authors: | Tomosugi, Wataru Browse this author | | Matsuda, Tomoki Browse this author | | Tani, Tomomi Browse this author | | Nemoto, Tomomi Browse this author | | Kotera, Ippei Browse this author | | Saito, Kenta Browse this author | | Horikawa, Kazuki Browse this author | | Nagai, Takeharu Browse this author |
| Issue Date: | May-2009 |
| Publisher: | Nature Publishing Group |
| Journal Title: | Nature Methods |
| Volume: | 6 |
| Issue: | 5 |
| Start Page: | 351 |
| End Page: | 353 |
| Publisher DOI: | 10.1038/NMETH.1317 |
| PMID: | 19349978 |
| Abstract: | We report a pH-insensitive and photostable ultramarine fluorescent protein, Sirius, with an emission peak at 424 nm, the shortest emission wavelength among fluorescent proteins reported to date. The pH-insensitivity of Sirius allowed prolonged visualization of biological events in an acidic environment. Furthermore, two FRET (fluorescence resonance energy transfer) pairs, Sirius-mseCFP and Sapphire-DsRed, allow dual-FRET imaging with single wavelength excitation, enabling detection of Ca^[2+] concentration and caspase-3 activation in the same apoptotic cells. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/39667 |
| Appears in Collections: | 電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 永井 健治
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