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An ultramarine fluorescent protein with increased photostability and pH insensitivity

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Title: An ultramarine fluorescent protein with increased photostability and pH insensitivity
Authors: Tomosugi, Wataru Browse this author
Matsuda, Tomoki Browse this author
Tani, Tomomi Browse this author
Nemoto, Tomomi Browse this author
Kotera, Ippei Browse this author
Saito, Kenta Browse this author
Horikawa, Kazuki Browse this author
Nagai, Takeharu Browse this author
Issue Date: May-2009
Publisher: Nature Publishing Group
Journal Title: Nature Methods
Volume: 6
Issue: 5
Start Page: 351
End Page: 353
Publisher DOI: 10.1038/NMETH.1317
PMID: 19349978
Abstract: We report a pH-insensitive and photostable ultramarine fluorescent protein, Sirius, with an emission peak at 424 nm, the shortest emission wavelength among fluorescent proteins reported to date. The pH-insensitivity of Sirius allowed prolonged visualization of biological events in an acidic environment. Furthermore, two FRET (fluorescence resonance energy transfer) pairs, Sirius-mseCFP and Sapphire-DsRed, allow dual-FRET imaging with single wavelength excitation, enabling detection of Ca^[2+] concentration and caspase-3 activation in the same apoptotic cells.
Type: article (author version)
Appears in Collections:電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 永井 健治

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