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Hertwig's epithelial root sheath cells do not transform into cementoblasts in rat molar cementogenesis

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Title: Hertwig's epithelial root sheath cells do not transform into cementoblasts in rat molar cementogenesis
Authors: Yamamoto, Tsuneyuki Browse this author →KAKEN DB
Takahashi, Shigeru Browse this author →KAKEN DB
Keywords: Cementoblasts
Hertwig's epithelial root sheath
Epithelial-mesenchymal transformation
Keratin
Runx2
Vimentin
Rat molars
Issue Date: 20-Nov-2009
Publisher: Elsevier
Journal Title: Annals of Anatomy
Journal Title(alt): Anatomischer Anzeiger
Volume: 191
Issue: 6
Start Page: 547
End Page: 555
Publisher DOI: 10.1016/j.aanat.2009.07.004
PMID: 19716687
Abstract: It is generally accepted that cementoblasts originate in the process of differentiation of mesenchymal cells of the dental follicle. Recently, a different hypothesis for the origin of cementoblasts has been proposed. Hertwig's epithelial root sheath cells undergo the epithelial-mesenchymal transformation to differentiate into cementoblasts. To elucidate whether the epithelial-mesenchymal transformation occurs in the epithelial sheath, developing rat molars were examined by keratin-vimentin and Runx2 (runt-related transcription factor 2)-keratin double immunostaining. In both acellular and cellular cementogenesis, epithelial sheath and epithelial cells derived from the epithelial sheath expressed keratin, but did not express vimentin or Runx2. Dental follicle cells and cementoblasts, however, expressed vimentin and Runx2, but did not express keratin. No cells showed coexisting keratin-vimentin or Runx2-keratin staining. These findings suggest that there is no intermediate phenotype transforming epithelial to mesenchymal cells, and that epithelial sheath cells do not generate mineralized tissue. This study concludes that the epithelial-mesenchymal transformation does not occur in Hertwig's epithelial root sheath in rat acellular or cellular cementogenesis and that the dental follicle is the origin of cementoblasts, as has been proposed in the original hypothesis.
Type: article (author version)
URI: http://hdl.handle.net/2115/39726
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山本 恒之

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