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Monoclonal Antibody against Bovine Lactoferricin® and Its Epitopic Site

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Title: Monoclonal Antibody against Bovine Lactoferricin® and Its Epitopic Site
Other Titles: Monoclonal Antibody against Bovine Lactoferricin(R) and Its Epitopic Site
Authors: Shimazaki, Kei-ichi1 Browse this author →KAKEN DB
Nam, Myoung Soo Browse this author
Harakawa, Shinji3 Browse this author
Tanaka, Tetsuya4 Browse this author →KAKEN DB
Omata, Yoshitaka5 Browse this author →KAKEN DB
Saito, Atsushi6 Browse this author →KAKEN DB
Kumura, Haruto7 Browse this author →KAKEN DB
Mikawa, Katsuhiko8 Browse this author
Igarashi, Ikuo9 Browse this author →KAKEN DB
Suzuki, Naoyoshi10 Browse this author →KAKEN DB
Authors(alt): 島崎, 敬一1
原川, 信二3
田仲, 哲也4
小俣, 吉孝5
齋藤, 篤志6
玖村, 朗人7
三河, 勝彦8
五十嵐, 郁夫9
鈴木, 直義10
Keywords: lactoferricin
monoclonal antibody
Issue Date: 25-Dec-1996
Publisher: 日本獣医学会
Journal Title: The Journal of Veterinary Medical Science
Volume: 58
Issue: 12
Start Page: 1227
End Page: 1229
Abstract: 牛ラクトフェリンのN-末端に近い25残基からなるペプチド,ラクトフェリシン (LFcin) は非常に強い抗菌活性を示す.LFcinの多様な機能の発現機作を解明する一手段として,抗LFcinモノクローナル抗体 (mAb) を作成した.LFcinはKLHにカップリングさせてマウスを免疫した.得られたmAbは化学合成したLFcinとも反応性を示した.膜上でのべプチド合成システムSPOTsによる判定結果,およびアミノ酸側鎖の化学修飾による結果と併せ,抗LFcin mAbの結合する部位はQWRと推定された.
Bovine lactoferricin® (LFcin B) is a strong antimicrobial peptide derived from N-lobe of lactoferrin. To study the immunochemical and structural properties of LFcin B, monoclonal antibody (mAb) was prepared and the amino acid sequence concerning with the binding to mAb has becn identified. Mice injected with LFcin B showed no production of antibody specific to this peptide, whereas those with LFcin B-KLH conjugate produced anti-LFcin B antibodies. None of the mAb reacted with bovine lactoferrin C-lobe, human lactoferrin or LFcin H. By the reactivity of the mAb against the peptides synthesized on cellulose membranes using SPOTs™ and against chemically modified derivatives of LFcin B, the antigenic determinant of LFcin B was identified to be the sequence of "QWR".
Rights: 本文データは学協会の許諾に基づきCiNiiから複製したものである日本獣医学会
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 島崎 敬一

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