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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
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Expressions of the multidrug resistance-related proteins in the rat olfactory epithelium : A possible role in the phase III xenobiotic metabolizing function
| Title: | Expressions of the multidrug resistance-related proteins in the rat olfactory epithelium : A possible role in the phase III xenobiotic metabolizing function |
| Authors: | Kudo, Hideaki Browse this author | | Doi, Yoshiaki Browse this author | | Fujimoto, Sunao Browse this author |
| Keywords: | Olfactory epithelium | | Multidrug resistance-related protein | | Xenobiotic metabolism | | Immunohistochemistry | | In situ hybridization | | Rat |
| Issue Date: | 4-Jan-2010 |
| Publisher: | Elsevier |
| Journal Title: | Neuroscience Letters |
| Volume: | 468 |
| Issue: | 2 |
| Start Page: | 98 |
| End Page: | 101 |
| Publisher DOI: | 10.1016/j.neulet.2009.10.073 |
| PMID: | 19879335 |
| Abstract: | The xenobiotic metabolizing system is considered to play important roles in the olfaction by the chemical homeostasis. Several phase I and phase II xenobiotic metabolizing enzymes are expressed in the olfactory epithelium in vertebrates. Multidrug resistance-related proteins (MRPs) are the phase III xenobiotic metabolizing pumps that eliminate some conjugated ligands from cells. However, the MRP-expressions in the olfactory epithelium have not been confirmed in the mammals. We investigated gene and protein expressions of MRP type 1 (MRP1) and type 2 (MRP2) isoforms in the adult rat olfactory epithelium in order to clarify the existence of phase III xenobiotic metabolizing pumps in the olfactory organs. Expressions of MRP1 mRNA were detected in the nasal cavity by reverse transcriptase polymerase chain reaction (RT-PCR). The nucleoside sequence of the RT-PCR products were completely identical to that found in other organs of rat. On the contrary, the analysis did not detect expressions of MRP2 mRNA in the nasal cavity. By in situ hybridization using a digoxigenin-labeled MRP1 cRNA probe, signals for MRP1 mRNA were observed preferentially in the perinuclear regions of supporting cells. However, the respiratory epithelial cells did not show the signals for MRP1 mRNA. By immunohistochemistry using a specific antibody to MRP1, MRP1-immunoreactivities were seen mainly on the supporting cells. These findings suggest that MRP1 is involved in olfaction as a part of the "olfactory signal termination" by the chemical homeostasis in the "perireceptor events" of the olfactory epithelium. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/39983 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 工藤 秀明
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