Structures of restriction endonuclease HindIII in complex with its cognate DNA and divalent cations


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Title:	Structures of restriction endonuclease HindIII in complex with its cognate DNA and divalent cations
Authors:	Watanabe, Nobuhisa Browse this author
	Takasaki, Yozo Browse this author
	Sato, Chika Browse this author
	Ando, Shoji Browse this author
	Tanaka, Isao Browse this author
Keywords:	restriction endonuclease
	DNA complex
	vaporizing iodine labeling method
Issue Date:	Dec-2009
Publisher:	International Union of Crystallography
Journal Title:	Acta Crystallographica. Section D, Biological Crystallography
Volume:	65
Issue:	12
Start Page:	1326
End Page:	1333
Publisher DOI:	10.1107/S0907444909041134
Abstract:	The three-dimensional crystal structures of HindIII bound to its cognate DNA with and without divalent cations were solved at 2.17 and 2.00 Å resolution, respectively. HindIII forms a dimer. The structures showed that HindIII belongs to the EcoRI-like (α-class) subfamily of type II restriction endonucleases. The cognate DNA-complex structures revealed the specific DNA-recognition mechanism of HindIII by which it recognizes the palindromic sequence A/AGCTT. In the Mg2+ ion-soaked structure the DNAwas cleaved and two ions were bound at each active site, corresponding to the two-metal-ion mechanism.
Type:	article
URI:	http://hdl.handle.net/2115/39986
Appears in Collections:	生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 渡邉信久

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