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Detection of UVBR-sensitive and -tolerant bacteria in surface waters of the western North Pacific

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Title: Detection of UVBR-sensitive and -tolerant bacteria in surface waters of the western North Pacific
Authors: Kataoka, Takafumi Browse this author
Hodoki, Yoshikuni Browse this author
Suzuki, Koji Browse this author
Saito, Hiroaki Browse this author
Higashi, Seigo Browse this author
Keywords: UVBR
Thymine dimer
Marine bacteria
Issue Date: 4-May-2009
Publisher: Elsevier B.V.
Journal Title: Journal of Photochemistry and Photobiology B : Biology
Volume: 95
Issue: 2
Start Page: 108
End Page: 116
Publisher DOI: 10.1016/j.jphotobiol.2009.02.004
PMID: 19285879
Abstract: In order to evaluate the effects of solar ultraviolet radiation (UVR) on eubacterial community composition, we examined the tolerance of eubacterial phylotypes to solar UV radiation in surface waters of the western North Pacific during September 2005. Bromodeoxyuridine (BrdU), a halogenated thymine analogue, was used for labeling newly synthesized DNA in proliferating cells. Thymine dimers (TD), which are specifically formed in DNA by biologically harmful ultraviolet B radiation (UVBR; 280-315 nm), were also applied to detect UVB damaged genomes selectively. PCR-denaturing gradient gel electrophoresis (PCR-DGGE) on the labeled samples revealed that UVBR-resistant cells showing active synthesis of DNA without accumulating TD, varied among phylotypes. In addition, UVBR-sensitive band positions with TD indicated inter-specific variations in sensitivity to UVBR. Phylogenetic analysis revealed that 12 DNA sequences were classified into 8 phylogenetic groups: 3 Rosecbacter, 1 Sphingomonas, 2 Gammaproteobacteria, 1 Actinobacteria, 1 Synechococcus, 2 Prochlorococcus, 1 plastid and 1 another group. A UVBR-resistant phylotype was affiliated to Erythrobacter sp. (previously designated as Sphingomonas sp.), which was distributed in warmer waters from the south of Oyashio to Kuroshio regions. A UVBR-sensitive phylotype was affiliated to Pseudoalteromonas sp. in Gammaproteobacteria. Dominant heterotrophic eubacteria were composed of both sensitive and resistant phylotypes. This is the first report on TD accumulated eubacterial phylotypes in oceanic surface waters.
Type: article (author version)
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 東 正剛

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